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OJVRTM
Online
Journal of Veterinary Research©
Volume 22
(6):444-451, 2018.
Effect
of platelet rich plasma on lamb spermatogonial stem
cell colonies.
Nooshin Kazemi,
Peyman Rahimi-Feyli, Ali-Asghar Moghaddam
Department
of Clinical Sciences, Faculty of Veterinary Medicine, Razi
University, Kermanshah, Iran. Corresponding author Email: Moghaddam@razi.ac.ir
ABSTRACT
Kazemi N, Rahimi-Feyli P, Moghaddam AA., Effect
of platelet rich plasma on lamb spermatogonial stem
cell colonies, Onl J Vet Res., 22 (6):444-451, 2018. Spermatogonial stem cells (SSC) provide
an excellent model system for differentiation, development and functioning of
testes and for production of transgenic animals. We describe effects of
different concentrations of Platelet-Rich Plasma (PRP) on SSCs colony
formation. Testicular cells were isolated from testes of prepubertal
lambs by enzymatic digestion then cultured for 10 days. Control cells were
grown in basic DMEM media with 1% Penicillin and streptomycin and 5% Fetal
Bovine Serum. Test SCC were treated with
1%, 2% or 4% PRP. Culture media were
changed every 72h. Cell colonies were assayed by inverted microscope and SSCs were
identified by immunocytochemistry assay against Protein Gene Product 9.5. Compared
with values at day 4, all colonies (treated and controls) increased by day 10
but there were no significant differences between SCC colonies and surface area
at days 4, 7 or 10 (P≥0.05). We
find that PRP does not affect SSC colonies in
vitro. On the contrary, high levels of PRP may inhibit SCC colonies and
surface area.
Key Words: Stem cell, Spermatogony, Colony formation, Platelet-Rich Plasma.
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