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OJVRTM

 

Online Journal of Veterinary Research

 

 Volume 22 (6):444-451, 2018.


Effect of platelet rich plasma on lamb spermatogonial stem cell colonies.

 

Nooshin Kazemi, Peyman Rahimi-Feyli, Ali-Asghar Moghaddam

 

Department of Clinical Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran. Corresponding author Email: Moghaddam@razi.ac.ir

 

ABSTRACT

 

Kazemi N, Rahimi-Feyli P, Moghaddam AA., Effect of platelet rich plasma on lamb spermatogonial stem cell colonies, Onl J Vet Res., 22 (6):444-451, 2018. Spermatogonial stem cells (SSC) provide an excellent model system for differentiation, development and functioning of testes and for production of transgenic animals. We describe effects of different concentrations of Platelet-Rich Plasma (PRP) on SSCs colony formation. Testicular cells were isolated from testes of prepubertal lambs by enzymatic digestion then cultured for 10 days. Control cells were grown in basic DMEM media with 1% Penicillin and streptomycin and 5% Fetal Bovine Serum. Test SCC were treated with 1%, 2% or 4% PRP. Culture media were changed every 72h. Cell colonies were assayed by inverted microscope and SSCs were identified by immunocytochemistry assay against Protein Gene Product 9.5. Compared with values at day 4, all colonies (treated and controls) increased by day 10 but there were no significant differences between SCC colonies and surface area at days 4, 7 or 10 (P≥0.05). We find that PRP does not affect SSC colonies in vitro. On the contrary, high levels of PRP may inhibit SCC colonies and surface area.

 

Key Words: Stem cell, Spermatogony, Colony formation, Platelet-Rich Plasma.


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