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Online Journal of Veterinary Research©
(Including Medical and Laboratory Research)
Volume 22 (11):1068-1076, 2018.
Effect of sirolimus and doxorubicin on aberrant colorectal crypt foci induced by azoxymethane in Balb/C male mice.
Eman S Hameed and Falah Muosa Kadhim AL-Rekabi
College of Veterinary Medicine, University of Baghdad.
Hameed ES, Kadhim AL-Rekabi FM., Effect of sirolimus and doxorubicin on aberrant colorectal crypt foci induced by azoxymethane in Balb/C male mice, Onl J Vet Res., 22 (11):1068-1076, 2018. Authors report effect of sirolimus and doxorubicin on aberrant crypt foci induced with azoxymethane (AOM) in Balb/C male mice. Aberrant colorectal crypt foci stem cells in 30 mice were induced by intraperitoneal (IP) administration of 10mg/kg Azoxymethane IP once weekly for 2 weeks. Groups of 10 mice each were then gavaged daily 34µg/kg sirolimus (Group 1) for 4 weeks or 50mg/m² Doxorubicin (Group 2) once weekly (after azoxymethane treatments), for 4 weeks. Cancer controls (Group 3) consisted of 10 mice given only AOM as described. Non-cancer controls (Group 4) were injected 2 ml saline IP daily for 4 weeks. Mice were sacrificed for tissue samples at day 28. Aberrant crypt foci (ACF) were counted by methylene blue and proliferation cell nuclear antigen (PCNA) by immunochemistry and scored as low 0-4 and high levels of expression ˃ 4-12. Compared with cancer controls (9.66 ± 1.20A), we found significant declines in ACF in mice given Sirolimus 2.66 + 0.33 or rapamycin 1.66 + 0.33, and PCNA (12 in cancer controls and 0.8 in treated mice). However we found no difference between treatments.
Key words: Sirolimus, Doxorubicin, Aberrant crypt foci, mice. Raw data provided.Key words: Sirolimus, Doxorubicin, Aberrant crypt foci, mice. Raw data provided.