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Online Journal of Veterinary Research©
(Including Medical and Laboratory Research)
Volume 25 (9):677-683, 2021.
Effects of Silymarin on liposaccharide induced toxicity in offspring of pregnant mice.
Amir Ali Shahbazfar PhD, Peyman Zare PhD, Maryam Mirzaee.
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
Shahbazfar AA, Zare P, Mirzaee M., Effects of Silymarin on liposaccharide induced toxicity in offspring of pregnant mice, Onl J Vet Res., 25 (9):677-683, 2021. Pre-parturition stress or toxicity affects offspring. Male and female balb-C mice were bred for offspring with pregnancy confirmed by vaginal plaque. Then, groups of 6 pregnant mice each were injected low 250 and high dose 500ug/kg liposaccharide (LPS) gavaged daily 0.5% silymarin for 5 days. Controls were not treated. Groups of 6 each offspring were injected LPS intraperitoneally (IP) at 67 days of age and blood taken 1.5hr after for determination of interleukin (IL-1β), tissue necrosis factor (TNF-α) and interleukin 6 (IL-6) measured by indirect ELISA. Another group of offspring were injected LPS IP at 67 days and sacrificed at 70 days for brain and liver tissue microscopy. Compared with pregnant controls, mice given LPS, IL6 increased 24% in pregnant and IL-1β 32% in pregnant and 49% in offspring (P 0.05), whereas in those given silymarin these values remained unchanged. We found no effect of LPS with or without silymarin on TNF-α in pregnant or offspring mice. By microscopy, in offspring, high maternal LPS doses induced marked inflammation in meninges and brain, scattered neuronal necrosis and gliosis and in liver, inflammation of portal area and necrosis of hepatocytes whereas in those given silymarin we observed milder histopathology. Our findings suggest maternal silymarin may protect offspring against liposaccharide LPS toxicity. Images are supplied to support findings.
Keywords: Silymarin; Mouse; LPS; Brain; Liver; Offspring, Raw data supplied.