©2021-2032 All Rights Reserved. Online Journal of
Veterinary Research. You may not store these pages in any form except for your
own personal use. All other usage or distribution is illegal under
International copyright treaties. Permission to use any of these pages in any
other way besides the before mentioned must be gained in writing from the
publisher. This article is exclusively copyrighted in its entirety to OJVR
publications. This article may be copied once but may not be, reproduced or
re-transmitted without the express permission of the editor.
OJVRTM
Online Journal of Veterinary Research©
(Including Medical
and Laboratory Research)
Established 1994
ISSN 1328-925X
Volume 25 (9):677-683, 2021.
Effects
of Silymarin on liposaccharide induced toxicity in offspring
of pregnant mice.
Amir
Ali Shahbazfar PhD, Peyman Zare PhD, Maryam Mirzaee.
Department of Pathobiology,
Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
ABSTRACT
Shahbazfar AA, Zare
P, Mirzaee M., Effects of Silymarin
on liposaccharide induced toxicity in offspring of pregnant mice, Onl J Vet Res., 25
(9):677-683, 2021. Pre-parturition stress or toxicity affects offspring. Male and female balb-C mice were bred for offspring with pregnancy
confirmed by vaginal plaque. Then, groups of 6 pregnant mice each were injected
low 250 and high dose 500ug/kg liposaccharide (LPS) gavaged
daily 0.5% silymarin for 5 days. Controls were not treated.
Groups of 6 each offspring were injected LPS intraperitoneally (IP) at 67 days
of age and blood taken 1.5hr after for determination of interleukin (IL-1β),
tissue necrosis factor (TNF-α) and interleukin 6 (IL-6) measured by
indirect ELISA. Another group of offspring were injected LPS IP at 67 days and sacrificed
at 70 days for brain and liver tissue microscopy. Compared with pregnant controls,
mice given LPS, IL6 increased 24% in pregnant and IL-1β 32% in pregnant and
49% in offspring (P 0.05), whereas in those given silymarin
these values remained unchanged. We found no effect of LPS with or without silymarin on TNF-α in pregnant or offspring mice. By
microscopy, in offspring, high maternal LPS doses induced marked inflammation in
meninges and brain, scattered neuronal necrosis and gliosis and in liver,
inflammation of portal area and necrosis of hepatocytes whereas in those given silymarin we observed milder histopathology. Our findings
suggest maternal silymarin may protect offspring
against liposaccharide LPS toxicity. Images are supplied to
support findings.
Keywords: Silymarin;
Mouse; LPS; Brain; Liver; Offspring, Raw data supplied.
FULL-TEXT (SUBSCRIBE OR
PURCHASE TITLE)