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Online Journal of Veterinary Research©

Volume 24 (5):284-289, 2020.

Effects of silymarin supplement on antibody titers in broilers vaccinated  against Newcastle and Avian influenza.


Hosseinian SA, Abdi-Hachesoo B, Hashemi Hazaveh SA


Department of Clinical Science, School of Veterinary Medicine, Shiraz University, Shiraz, Iran




Hosseinian SA, Abdi-Hachesoo B, Hashemi Hazaveh SA., Effects of  silymarin supplement on antibody titers in broilers vaccinated  against Newcastle and Avian influenza, Onl J Vet Res., 24 (5):284-289, 2020. We describe effect of silymarin supplement given with mash or pellet diets on antibody titers in broilers injected Newcastle and avian flu vaccines. Groups of 20 day old Arbor Acre chicks were given basal mash, pellet with or without 500 or 2500ppm silymarin diets daily for 42 days. Birds were vaccinated Newcastle via eye drop at Day 8 with Hichner B1 strain, and subcutaneous inactivated Newcastle and Influenza La Sota with H9N2 strains. Blood was taken at 21, 28, 35 and 42 for antibody titers. Compared with mash and pellet controls we found ~21-46% and ~22-41% increases (P < 0.05), respectively, in Newcastle virus disease antibody titers in broilers given 500-2500 ppm silymarin supplements. Concerning avian flu antibody titers, compared with mash controls, we found no change in antibody titers in birds given 500ppm silymarin . However in those given 2500ppm same titers increased (P < 0.05) ~28-42%. Compared with pellet controls avian flu titers increased 17-40% in those given 500-2500 ppm silymarin.  Antibody titers for both vaccines peaked by 28 and 35 days in all birds. Results suggest that silymarin may enhance antibody titers to Newcastle’s and avian flu vaccine. Greater pellet digestibility compared with mash may have increased availability of silymarin.     


Key words: broilers, diet forms, immune response, silymarin.