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 OJVRTM

 

Online Journal of Veterinary Research  

(Including Medical and Laboratory Research)

Established 1994

ISSN 1328-925X

 

Volume 28 (2): 71-76, 2024.


Synthesis and properties of de-celled sheep omentum soft-tissue scaffold.

 

Ahmed Ali Khirallah1, Bahaa Fakhri Hussein2

 

1. College of Veterinary Medicine, University of Baghdad/Iraq, 2. Tissue Engineering , University of Baghdad/Iraq

 

ABSTRACT

 

Khirallah AA, Hussein BF., Synthesis and properties of de-celled sheep omentum soft-tissue scaffold., Onl J Vet Res., 28 (2): 71-76, 2024., We describe creation and use of sheep omentum for in vitro soft-tissue engineering scaffolds wherein metabolism induces new tissue. A porous scaffold was designed using connective tissue from sheep omentum de-celled by freeze-thaw cycle and disinfected with 70% alcohol at -40ᵒC to form intracellular crystals bound to DNA. Properties of scaffold were confirmed by glycosaminoglycan, decellularization and mechanical tests, and scanning electron microscopy. Extracellular matrix consisted of disaccharide glycosaminoglycan, chondroitin and dermatan sulfates. At room temperature, samples were packed in airtight polyethylene bags, sealed, and sterilized by gamma irradiation. Scxanning electron microscopy confirmed de-celling, removal of lipids and nuclei.

 

Keywords: Omentun Scaffold, stem cells, ovine.


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