2023-2035 All Rights Reserved. Online Journal of Veterinary Research . You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the  before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to OJVR.This article may be copied once but may not be, reproduced or  re-transmitted without the express permission of the editors. This journal satisfies the refereeing requirements (DEST) for the Higher Education Research Data Collection (Australia). Linking:To link to this page or any pages linking to this page you must link directly to this page only here rather than put up your own page.



Online Journal of Veterinary Research  

(Including Medical and Laboratory Research)

Established 1994

ISSN 1328-925X


Volume 28 (2): 71-76, 2024.

Synthesis and properties of de-celled sheep omentum soft-tissue scaffold.


Ahmed Ali Khirallah1, Bahaa Fakhri Hussein2


1. College of Veterinary Medicine, University of Baghdad/Iraq, 2. Tissue Engineering , University of Baghdad/Iraq




Khirallah AA, Hussein BF., Synthesis and properties of de-celled sheep omentum soft-tissue scaffold., Onl J Vet Res., 28 (2): 71-76, 2024., We describe creation and use of sheep omentum for in vitro soft-tissue engineering scaffolds wherein metabolism induces new tissue. A porous scaffold was designed using connective tissue from sheep omentum de-celled by freeze-thaw cycle and disinfected with 70% alcohol at -40ᵒC to form intracellular crystals bound to DNA. Properties of scaffold were confirmed by glycosaminoglycan, decellularization and mechanical tests, and scanning electron microscopy. Extracellular matrix consisted of disaccharide glycosaminoglycan, chondroitin and dermatan sulfates. At room temperature, samples were packed in airtight polyethylene bags, sealed, and sterilized by gamma irradiation. Scxanning electron microscopy confirmed de-celling, removal of lipids and nuclei.


Keywords: Omentun Scaffold, stem cells, ovine.