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Online Journal of Veterinary Research


Volume 26 (6): 402-412, 2022.

Reference plasma selenium, zinc, manganese, copper and antioxidants in grazing pregnant ewes


M. Mohebbi-Fania, A. Mirzaeib, S. Nazifib* , M. R. Tabandehc, Z. Shabbooeid


aDepartment of Animal Health Management, bClinical Studies, School of Veterinary Medicine, Shiraz University, Shiraz; cDepartment of Basic Sciences, School of Veterinary Medicine, Shahid Chamran University, Ahvaz, dSchool of Veterinary Medicine, Shiraz University, Shiraz, Iran




Mohebbi-Fani M, Mirzaei A, Nazifi S, Tabandeh MR, Shabbooei Z., Reference plasma selenium, zinc, manganese, copper and antioxidants in grazing pregnant ewes, Onl J Vet Res., 26 (6): 402-412, 2022. Reference plasma copper (Cu), zinc (Zn), manganese (Mn), selenium (Se) and antioxidant enzymes were determined in pregnant fat tailed grazing ewes. Whole blood samples of 47 ewes were taken at the first day of a 51-day breeding period (before pregnancy) and 120 days later (70-120 days of pregnancy). Pregnancy was confirmed in 43 ewes by plasma progesterone at day 120. For the whole period of the study, the ewes were grazing on medium-to-low quality pastures and cereal stubble and were offered about 1 kg/head/day of a mixture of alfalfa hay (40%) and wheat straw (60%) plus at least 100g barley grain/head/day. A trace mineralized supplement was available free choice. We measured erythrocyte malondialdehyde, superoxide dismutase, glutathione peroxidase and plasma copper, zinc manganese and selenium. In pregnant ewes malondialdehyde increased ~33% (P≤0.05) at day 120 suggesting oxidative stress whereas superoxide and gluthathione oxiudasa, copper and zinc declined ~41-62%. Findings suggest ewes experience oxidative stress during pregnancy when fed medium to low quality forages.


Key words: Pregnancy, Selenium, Zinc, Copper, Superoxide dismutase, Glutathione peroxidase, Oxidative stress, Sheep.