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OJVRTM
Online
Journal of Veterinary Research©
Volume 17 (6): 317-322, 2013. Redacted 2017
Duration of replication
and shedding of H9N2 virus from the trachea and
feces of intranasally inoculated broilers.
Hadi Tavakkoli1
and Reza Kheirandish2
1Department(s) of Avian Medicine and 2Pathobiology, School of Veterinary Medicine, Shahid
Bahonar University of Kerman, Kerman, Iran
ABSTRACT
Tavakkoli H, Kheirandish R., Duration of
replication and shedding of H9N2 virus in the trachea and feces of broilers, Onl J Vet Res., 17 (6): 317-322, 2013. H9N2 avian
influenza subtype is endemic in chickens in Asia and is a threat to humans
because of affinity with alpha-2, 6-linked sialic acid receptors in the human
upper respiratory tract. The duration of H9N2 viral replication and shedding from
trachea and
feces of chickens after nasal inoculation is reported. 25 day-old commercial
broiler chicks were inoculated intra-nasally with low pathogenic
A/Chicken/Iran/SH-110/99(H9N2) virus. Titers were determined using TaqMan real time PCR after inoculation. Virus was detected
in trachea 3 to 7 days and in feces up to 11 days. Maximum titers occurred on day 7 with 8876 copies in trachea and 3353919 in
feces. The findings suggest that birds could pose a threat to humans for 3 to 11 days during an avian influenza
outbreak.
Keywords: Chicken, H9N2, TaqMan
real time PCR, Shedding.
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