©1996-2009 All Rights Reserved.
Online Journal of Bioinformatics . You may not store
these pages in any form except for your own personal use. All other usage or distribution
is illegal under international copyright treaties. Permission to use any of
these pages in any other way besides the before
mentioned must be gained in writing from the publisher. This article is
exclusively copyrighted in its entirety to OJB publications. This article may
be copied once but may not be, reproduced or re-transmitted
without the express permission of the editors. This journal satisfies the refereeing requirements
(DEST) for the Higher Education Research Data Collection (Australia). Linking:To link to this page or
any pages linking to this page you must link directly to this page only here
rather than put up your own page.
OJBTM
Online Journal of Bioinformatics©
Volume 8 (1):75-83, 2007
In silico Cis-regulatory Elements Analysis of Seed Storage
Protein Promoters Cloned
from Different Cultivars of Wheat, Rice and Oat
Yadav D1, Singh VK1, Singh NK2
1Department of Molecular Biology and Genetic Engineering,
ABSTRACT
Yadav D, Singh VK, Singh
NK., In
silico Cis-regulatory Elements Analysis of Seed Storage
Protein Promoters Cloned from Different Cultivars of Wheat, Rice and Oat, Online
J Bioinformatics, 8 (1):75-83, 2007. A
total of 24 promoter sequences with assigned accession number EF393165 to
EF393188 and representing major seed storage proteins of wheat namely High
molecular weight glutenin subunit (HMW-GS), low
molecular weight glutenin subunits (LMW-GS)
alpha/beta gliadins, triticin
along with rice glutelins and oat 12S globulins were
cloned from indigenous cultivars of wheat, rice and oat and was subjected to in silico
analysis using bioinformatic softwares
for the presence of different cis-regulatory motifs. The phylogeny studies based on the
multiple sequence alignment of these promoters revealed four distinct clusters
showing major group of seed storage promoters. The presence of additional
motifs like RY repeats, ABRE, AC-11, CAAT box, LTR, UTR, CCGTCC box, G box,
GARE, MBS along with the common motifs present in seed storage promoters like Prolamin-box, TATA, CAAT provides a better option for
multifarious uses.
Keywords: Seed storage protein promoters, Cis-regulatory
Elements, In silico.