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Online Journal of Veterinary Research©
(Including Medical and Laboratory Research)
Volume 22 (12):1137-1144, 2018.
Single cell protein production by soil Raoutella ornithinolytica incubated on waste potato, paper and corn cob media.
*Oqbah Abdul Halim Abdul Jabbar Al-Hadithi, *Dhafer Fakhri Al-Rawi, **Mohammed Qais Abed Al-Ani
* College of Education for Pure Sciences, **College of Science, Anbar University, Iraq.
Al-Hadithi OAH, Al-Rawi DF, Abed Al-Ani MQ., Single cell protein production by soil Raoutella ornithinolytica incubated on waste potato, paper and corn cob media, Onl J Vet Res., 22 (12):1137-1144, 2018. We describe single cell protein (SCP) production characteristics by Raoutella ornithinolytica bacteria isolated from soil and cultured on processed potato, paper and corncob starch waste residue. We identified 3 strains (A1-3) of R. ornithinolytica by colony characteristics, microscopy and Kliger, urease, citrate, indole, catalalase, APUI 20, Vitek2 and motility tests finding that A1 was most productive. By inoculating 0.5ml/100ml R. ornithinolytica A1 on 10g/100 ml potato waste media for 72 hours at 50C and pH 8.5 we obtained an SCP with 24.4% protein, 24.6% carbohydrate 17.9% fat, 21.8% ash with humidity of 88.6%. DNA content was 1.235% and RNA 1.012%. Essential and non-essential amino acids constituted 93.9 mg/100g SCP. Copper constituted 0.002, phosphorus 3.90, zinc 0.00056, calcium 0.085, potassium 0.132 and sodium 3.86% in SCP.
Key words: single cell protein, Raoutella ornithinolytica