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OJVRTM
Online Journal of Veterinary
Research©
(Including Medical and Laboratory
Research)
Established 1994
ISSN 1328-925X
Volume 22 (12):1137-1144, 2018.
Single cell protein production by soil Raoutella
ornithinolytica incubated on waste potato, paper
and corn cob media.
*Oqbah
Abdul Halim Abdul Jabbar Al-Hadithi,
*Dhafer Fakhri
Al-Rawi, **Mohammed Qais
Abed Al-Ani
* College of Education
for Pure Sciences, **College of Science, Anbar University, Iraq.
ABSTRACT
Al-Hadithi OAH, Al-Rawi DF, Abed Al-Ani MQ., Single cell protein production by soil Raoutella ornithinolytica
incubated on waste potato, paper and corn cob media, Onl
J Vet Res., 22 (12):1137-1144, 2018. We
describe single cell protein (SCP) production characteristics by Raoutella ornithinolytica
bacteria isolated from soil and cultured on processed potato, paper and
corncob starch waste residue. We identified 3 strains (A1-3) of R. ornithinolytica by colony characteristics, microscopy
and Kliger, urease, citrate, indole, catalalase, APUI 20, Vitek2 and motility tests finding that
A1 was most productive. By inoculating 0.5ml/100ml R. ornithinolytica
A1 on 10g/100 ml potato waste media for
72 hours at 50C and pH 8.5 we obtained an SCP with 24.4% protein, 24.6% carbohydrate
17.9% fat, 21.8% ash with humidity of 88.6%. DNA content was 1.235% and RNA
1.012%. Essential and non-essential amino acids constituted 93.9 mg/100g SCP. Copper
constituted 0.002, phosphorus 3.90, zinc 0.00056, calcium 0.085, potassium 0.132
and sodium 3.86% in SCP.
Key words: single cell protein, Raoutella
ornithinolytica
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