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OJVRTM
Online
Journal of Veterinary Research©
Volume 21(5):223-233, 2017.
In Vitro 3-D human Wharton's jelly
scaffold for embryonic stem cells.
Bahaa Fakhri Hussein1* Khayreia Kazzum
Habeeb2
1Department(s) of Anatomy & Histology, 2Surgery, Faculty of Veterinary Medicine, University
of Baghdad, Baghdad, Iraq *Vice-Dean of Postgraduate and Scientific Affairs
ABSTRACT
Hussein BF, Habeeb KK., In Vitro 3-D human Wharton's jelly scaffold for
embryonic stem cells,Onl J
Vet Res., 21(4):223-233, 2017. Wharton's jelly (WJ) is a mucous
connective extra-embryonic mesoderm used as a postnatal source for fetal stem cells. An in vitro 3-D Wharton's jelly
scaffold made of human umbilical cord seeded with mesenchymal stem cells is
described. Mesenchymal stem cells in scaffold (1 x 107 ml) were
incubated in CO2, 37˚C and 90% humidity for 1h and 10ml media
was added to each flask, incubated again for further cell attachment. Seeded
samples were taken from freeze dried scaffolds sputter coated with
gold for micro structural characterization and particle size analysis by
scanning electron microscopy. We find that cells proliferated at different
rates in scaffolds. Results suggest that the scaffolds appeared similar to 5 to
10 day egg-embryo divisions: by 15 days of culture, myocardiac
muscle, fibrous and adipose tissue, and vascular blood vessels were present.
Key word: Umbilical Cord, Mesenchymal Stem
Cells, Wharton’s Jelly Scaffold.
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