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OJVRTM
Online Journal of Veterinary Research©
Volume 21(1):19-24, 2017.
Effect of Allium
sativum garlic oil on Escherichia coli O157:H7.
Mohammad Mahdi Zangeneh*1,2, Fariba Najafi2,
Reza Tahvilian3, Lida Haghnazari4,
Akram Zangeneh2,5,
Rohalah
Moradi2, Ali Mahmoudi Far2.
1Department(s) of
Clinical Sciences, 5Microbiology, Pathobiology and Basic sciences,
Faculty of Veterinary Medicine, Razi University, 2Dermatology,
3Pharmaceutics and 4Biochemistry, School of Medical
Sciences, Kermanshah, 5Microbiology, Pathobiology & Basic
sciences, Veterinary Faculty, Razi University,
Kermanshah, Iran.
ABSTRACT
Zangeneh MM, Najafi
F, Tahvilian R, Haghnazari
L, Zangeneh A, Moradi R,
Far AM., Effect of Allium sativum garlic oil
Escherichia coli O157:H7, Onl J Vet Res., 21(1):19-24,
2017. Effect of Allium sativum
oil (AS) on growth of the common pathogen Escherichia
coli O157:H7 in macro-dilution Mueller-Hinton broth agar disk or agar well
diffusion is described. The widest zone of inhibition in agar diffusion (20mm)
and agar well (14mm) occurred at 0.062 g/ml AS. Minimum inhibitory and
bactericidal concentrations were ~0.007 g/ml AS. There was no inhibition at
0.002 g/ml or in dimethyl sulfoxide (DMSO). We
find discrepancies in EC sensitivity to AS essential oil with greater
inhibition in disk diffusion tests.
Keywords: Allium sativum, Essential oil,
Antibacterial effect, Macro-dilution method, Agar well diffusion method, Agar
disk diffusion method.
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