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OJVRTM

Online Journal of Veterinary Research

 

Volume 23 (7):688-693, 2019.


Molecular phylogenetic analysis of equine sarcoid.

 

Mohammad Hamid Kareem1, Sufyan Saleh Salman2.

 

1Department(s) of Internal & Preventive Medicine, College(s) of Veterinary Medicine, University of Faluga, 2University of Baghdad, Iraq.

ABSTRACT

 

Kareem MH, Salman SS., Molecular phylogenetic analysis of equine sarcoid, Onl J Vet Res., 23 (7): 688-693, 2019. Authors report prevalence and phylogeny of sarcoid in 100 horses, selected from a cohort of 1262 suspect with skin lesions. We found incidence of ~7.8% with lesions mostly located on the head and neck, perineal region under the chin and around eyes. Multiplex PCR for genotyping 2 L-major capsid protein primers showed all to be BPV-1-L1 gene, none for BPV-2-L2 or BPV-13-L1. Amplification of DNA fragments by agarose gel electrophoresis showed a 718bp segment for nucleotide in L1 in BPV1 gene. DNA of bovine papillomavirus was found in 96 samples or 94.28% of 100 wart specimens again revealing a DNA 718bp segment with BPV1 specific primers. Our results replicate previous findings.

 

Key word: Equine sarcoid, (BPV-1/2), (BPV-13), types, PCR.


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