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Online Journal of Veterinary Research©
(Including Medical and Laboratory Research)
Volume 22 (11):1037-1047, 2018.
Properties of lipopolysaccharide from S259, virulence genes and antibiotic resistance in
Salmonella typhimurium isolated from 201 patients with diarrhea.
1WQ Moutab*, 2LM Aziz, 3Ibrahim A Abdul-Rahman.
1College of Education for Pure Science, University of Anbar,2College of Veterinary Medicine, University of Fallujah, 3College of Applied Sciences, University of Fallujah, Iraq.
Moutab WQ, Aziz LM, Abdul-Rahman IA, Properties of lipopolysaccharide from S259, virulence genes and antibiotic resistance in Salmonella typhymurium isolated from 201 patients with diarrhea, Onl J Vet Res., 22 (11):1037-1047, 2018. Salmonella spp were isolated from stools of hospital patients with diarrhea by standard and Vitec compact 2 methods. Seven of 15 were identified as S. thyphimurium. All strains possessed the invA gene (211pb), seven STM4497 (523pb) and three the Stn (617pb) gene. All isolates were highly resistant to rifampin and doxycycline 95-100%, but sensitive to amikacine, imipenem, aztreonam and ciproflaxacin (94-100%). However, isolate S259 was resistant to all antibiotics and had the 3 genes and was therefore used for LPS extraction. After partial purification the concentration of LPS in phenolic phase was 435.57µg/ml and aqueous phase 381.76 µg/ml equivalent to MW 93325 and 71614 Dalton.
Key words: Extraction, Lipopolysaccharide, Salmonella typhimurium, PCR