1994-2020. All Rights Reserved. Online Journal of Veterinary Research. You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to OJVR publications. This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editors.


OJVRTM

Online Journal of Veterinary Research

(Including Medical and Laboratory Research)

Established 1994

ISSN 1328-925X

 

Volume 22 (11):1037-1047, 2018.


Properties of lipopolysaccharide from S259, virulence genes and antibiotic resistance in

Salmonella typhimurium isolated from 201 patients with diarrhea.

 

1WQ Moutab*, 2LM Aziz, 3Ibrahim A Abdul-Rahman.

 

1College of Education for Pure Science, University of Anbar,2College of Veterinary Medicine, University of Fallujah, 3College of Applied Sciences, University of Fallujah, Iraq.

 

ABSTRACT

 

Moutab WQ, Aziz LM, Abdul-Rahman IA, Properties of lipopolysaccharide from S259, virulence genes and antibiotic resistance in Salmonella typhymurium isolated from 201 patients with diarrhea, Onl J Vet Res., 22 (11):1037-1047, 2018. Salmonella spp were isolated from stools of hospital patients with diarrhea by standard and Vitec compact 2 methods. Seven of 15 were identified as S. thyphimurium. All strains possessed the invA gene (211pb), seven STM4497 (523pb) and three the Stn (617pb) gene. All isolates were highly resistant to rifampin and doxycycline 95-100%, but sensitive to amikacine, imipenem, aztreonam and ciproflaxacin (94-100%). However, isolate S259 was resistant to all antibiotics and had the 3 genes and was therefore used for LPS extraction. After partial purification the concentration of LPS in phenolic phase was 435.57g/ml and aqueous phase 381.76 g/ml equivalent to MW 93325 and 71614 Dalton.

Key words: Extraction, Lipopolysaccharide, Salmonella typhimurium, PCR


MAIN

 

FULL-TEXT (SUBSCRIBE OR PURCHASE TITLE)