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Online Journal of Veterinary Research

 Volume 23 (11):1137-1144, 2019.

Effect of phenobarbital and chloramphenicol on toxicity of salinomycin LD50 dose in Ross broilers and layers.


H Rajaiana*, S Nazifib , M Aberumandia , J Jalaeia


aDepartments of Pharmacology and bClinical Pathology, School of Veterinary Medicine, Shiraz University, Shiraz , Iran




Rajaian H, Nazifi S, Aberumandi M, Jalaei J., Effect of phenobarbital and chloramphenicol on toxicity and LD50 of salinomycin in chickens, Onl J Vet Res., 23 (11):1137-1144, 2019. We determined mortality in broilers and layers given salinomycin at a dose equal to LD50 after administration of chloromphenicol and phenobarbital. Two and 4 days before oral salynomycin three groups of 17 broiler and 22 broilers each were injected IM 2 chloramphynecol (group 1) with phenobarbital (2) or saline (3). Wing vein blood was taken at 1, 3 and 5 days after salinomycin for serum creatinine, aspartate and alanine transaminases, calcium, sodium, potassium, chloride and glucose. LD50s of salinomycin in broiler chickens was ~108 and in layers ~104 mg/kg body weight, respectively. Compared with controls we observed reduced mortality (~9%) in layers given chloramphenicol or phenobarbital and broilers dosed chloramphenicol (~15%). However, in broilers given phenobarbital mortality was reduced by a much higher ~32%. There were no significant changes in biochemical parameters in broilers or layers. The LD50s of salinomycin in broiler chickens and laying hens obtained in this study are more or less similar to the values reported in the literature. The lowering of the death rate by phenobarbital in broiler chickens receiving LD50 dose of salinomycin is probably due to the inducing effect of phenobarbital on liver microsomal enzymes.


Key words: Salinomycin , LD50 , Chickens, Chloramphenicol, Phenobarbital.