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Online Journal of Veterinary Research©
Volume 23 (1):23-31, 2019.
Effect of saffron (Crocus sativus L.) extract on MCF-7 and MCF-10A breast cancer cells In Vitro.
Eskandari R1, Fazeli M2*, Ghahramani Seno MM3,
Department(s) of 1Biotechnology, 2Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, 3Department of Basic Sciences, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
Eskandari R, Fazeli M, Ghahramani Seno MM., Cytotoxic effect of saffron (Crocus sativus L.) extract on MCF-7 and MCF-10A breast cancer cells, Onl J Vet Res., 23 (1):23-31, 2019. MCF-7 cells were cultured in RPMI 1640 and MCF10A cells in DMEM/F12 media incubated with 1000 to 10000 μg/ml Crocus sativus L. saffron extract to determine IC50. HPLC chromatogram of the extract revealed 21.3 trans-crocin mg/100ml, 10.2 cis-crocin, 9.7 picrocrocin, 4.6 crocetin, and 2.4 safranal mg/100ml as major compounds with variable amounts of α-pinen, echinocystic acid, cineole, 1,8-cineol, 3-gentiobiosyl-kaempferol and oleanolic acid. Cell viability was assessed by MTT assay and trypan blue staining. The IC50 for MCF-7 cells treated by saffron extract at 24h was 6700ug/ml, 48h 3300 μg/ml and 72h 2200 μg/ml and for MCF-10A 7100, 4100, 3200 µg/ml.
Keywords: Crocus sativus, Cytotoxicity, Saffron, MCF-7, Breast cancer, MCF-10A.