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Online Journal of Veterinary Research

(Including Medical and Laboratory Research)

Established 1994
ISSN 1328-925X


Volume 27 (8): 496-502, 2023

Prevalence of Ehrlichia ruminantium by pCS20-PCR in sheep, goats cattle and camels in Sudan.


Ibrahim MB1, Saeed EMA2, Hassan SM12, Gameel AA2, Suleiman KM2, Zaki AS2.


1Ministry of Animal Resources and Fisheries, 2Faculty of Veterinary Medicine, University of Khartoum, Sudan.




Ibrahim MB, Saeed EMA, Hassan SM, Gameel AA, Suleiman KM, Zaki AS., Prevalence of Ehrlichia ruminantium by pCS20-PCR in sheep, goats cattle and camels in Sudan, Onl J Vet Res., 27 (8):496-502, 2023. We report outcomes of a survey for heartwater disease due to Ehrlichia ruminantium in 100 sheep, 40 goats, 20 cattle and 10 camels in Sudan determined by pCS20-PCR. Blood was spotted on filter paper for DNA extracted and amplified with HH1F and HH2R primers for 980bp fragment of E. ruminantium pCS20 gene. Our PCR was highly sensitive and specific for Ehrlichia ruminantium in symptomatic and asymptomatic carrier animals. Of 170 animal samples we found very low incidence (1.8%) for Ehrlichia ruminantium. Amblyomma lepidum. However, use of oxytetracycline for infections and acaracides may account for low incidence. Our results simulate previous findings in ticks with prevalence determined by pCS20-PCR with 1.8% in A. lepidum and 8.2% A. variegatum in Sudan. Our results suggest pCS20 PCR detected E. ruminantium in asymptomatic and symptomatic animals.


Key words: heartwater, Ehrlichia ruminantium pCS20-PCR, Sudan.