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Online Journal of Veterinary Research©

Volume 11(2):38-43, 2007. Redacted 2018.


Phenyl phosphorodiamidate (PPD) adversely affects electrophysiology of rumen epithelium in sheep

 

Abdoun K1,  Martens H2

 

1Department of Physiology, Faculty of Veterinary Medicine, University of Khartoum, 13314 Shambat, Sudan 2Institute of Veterinary Physiology, Faculty of Veterinary Medicine, Free University of Berlin, Oertzenweg 19b, 14163 Berlin, Germany

 

ABSTRACT

 

Abdoun K,  Martens H, Phenyl phosphorodiamidate (PPD) adversely affects the electrophysiology of the rumen epithelium in Sheep, Onl J Vet Res 11(2):38-43, 2007. The effect of mucosal application of 1mmol/l phenyl phosphorodiamidate (PPD) on the electrophysiology of isolated rumen epithelium was studied in vitro using a conventional Ussing chamber technique. Thirty-six isolated rumen epithelium samples from 5 sheep of different breeds, age and sex and solely fed on hay were used. Mucosal application of phenyl phosphorodiamidate, which has an application in improving the utilization of dietary urea in ruminants nutrition, on the mucosal side of ruminal epithelial tissues resulted in a significant (p<0.05) reduction of the transepithelial potential difference (PDt) and epithelial short circuit current (Isc), and a significant (p<0.05) elevation of the epithelial tissue conductance (Gt). Phenyl phosphorodiamidate could thus impair the barrier and transport function of the rumen epithelium.

 

KEY-WORDS: Electrophysiology; Phenyl phosphorodiamidate; Rumen; Sheep.


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