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OJVRTM
Online Journal of Veterinary Research©
Volume 19(5): 306-316, 2015.
Clarified rumen fluid as a source
of cellulase extraction by
Cibacron Blue coated magnetic
nanoparticles
Seyed Azizollah
Ghotb1, Mohammad Chamani1*, Homayon
Ahmadpanahi2, Ali Asghar Sadeghi1
1Faculty of Agriculture and Natural Resources,
Tehran Science and Research
Branch, Islamic Azad University, 2Chemistry, Central Tehran Branch, Islamic
Azad University, Tehran, Iran
ABSTRACT
Ghotb SA, Chamani M, Ahmadpanahi H, Sadeghi AA., Clarified rumen fluid as a source of cellulase extraction by Cibacron Blue coated magnetic nanoparticles, Onl J vet Res., 19(5): 306-316, 2015. Cellulase adsorption desorption from clarified rumen fluid with Cibacron Blue F3GA coated Magnetic iron oxide nanoparticles were modified by chemical co-precipitation. Size and morphology of particles were determined by transmission electron microscopy. The diameter of the spherical magnetic nanoparticles was 20-30nm. Modifications were confirmed by Fourier transform infrared and thermal gravimetric analysis. Optimized adsorption parameters for pH and temperature yielded cellulase adsorption of 53% at pH 6 and 45C. Desorption was performed with 0.1, 0.5 and 1M NaCl shaking for 15 or 90 min. The highest desorption (44%) was obtained with 1M NaCl with 90 min shaking.
Key
Words: Cellulase enzyme, Clarified rumen fluid, Cibacron
Blue, Magnetic nanoparticles.
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