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OJVRTM
Online Journal of
Veterinary Research©
Volume
3: 1-14, 1999. Redacted 2018.
Molecular characterization of porcine
rotavirus strains
Solarte Y1,
Correa A1, Barrera J2, Mogollon D2, Jerabek L1, Gutierrez MF1
1 Universidad Javeriana, 2 Corporación Colombiana de Investigaciones Agropecuarias, Instituto Colombiano Agropecuario. For
correspondence please contact:Maria
Fernanda Gutierrez. Departamento de Microbiología, Cra 7 # 43-82. Edificio Felix Restrepo Of. 111. Phone: (57)1 320 8 320, FAX (57)1
285 05 03. E-mail: mfgutier javercol.javeriana.edu.co
SUMMARY
Solarte Y, Correa
A, Barrera J, Mogollon D, Jerabek L, Gutierrez MF.,
Molecular characterization of porcine rotavirus strains Onl
J Vet Res., 3: 1-14, 1999. One hundred
thirty four diarrhea samples from suckling pigs between 5 and 29 days old were
collected within a one year period between June 1995 and June 1996. Forty-one
(31%) of the 134 were Rotavirus (RTV) positive by polyacrylamide gel
electrophoresis (PAGE) analysis, and all were identified as group A with short
migration patterns. The analysis demonstrated the presence of four electropherotypes. From among all of the positive samples,
9 were selected at random, to be grown in MA-104 cells, and 22 samples were
selected for electron microscope examination. Successful adaptation to cell
culture was obtained with two of the field isolates and the 5 reference strains
which were used as procedural controls. The viral serotyping was carried out by
means of RT-PCR and the serotype was found to be G5.
KEY WORDS:
Rotavirus, Electropherotypes, Viral Serotyping.
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