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Online Journal of Veterinary Research©
Volume 23 (1):56-65, 2019.
Expression of MicroRNA’s miR-10a, miR-214 and miR-29b as markers of fertility in roosters with normal and low sperm motility.
SH Valipour, AA Masoudi, R Vaez Torshizi.
Department of Animal Science, Faculty of Agriculture, Tarbiat Modares University, Tehran – Iran.
Valipour SH, Masoudi AA, Vaez Torshizi R., Expression of MicroRNA’s miR-10a, miR-214 and miR-29b as markers of fertility in roosters with normal and low sperm motility, Onl J Vet Res. 23 (1):56-65, 2019. In poultry, selection of fertile or infertile males is done by assessment of sperm motility and morphology, a method which bears weak correlation with fertility. However, microRNA’s (miRNA’s) which regulate gene expression, play a critical role in spermatogenesis. To our knowledge, this is a first report of expression of miRNAs, miR-10a, miR-214, and miR-29b and sperm motility in birds by RT-PCR. Predicted targets of the miRNAs involved in spermatogenesis were identified by in silico search in miRDB, TargetScan and DIANA microT uploaded into Enricher for gene ontology analysis. Protein-protein interaction (PPI) enrichment network constructed from STRING database revealed 77 nodes, 81 edges with a PPI p-value of 9.69e-08. The network showed 12 major significant (P < 0.05) organic cyclic compound and binding nodes (P < 0.05) molecular function pathways involved with spermatogenesis. However, we found that there was no correlation between expression of the 3 miRNAs with motility of sperm (P> 0.05) in this study. We find that miRNA’ are probably not useful markers of sperm motility.
Keywords: MicroRNA; Fertility, Roosters.