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Online Journal of Veterinary Research©
(Including Medical and Laboratory Research)
Volume 26 (11): 810-814, 2022.
Endogenous retrovirus subtypes in pigs.
M Suji Prabha MSc PhD, Susan Verghese MD PhD.
Department of Microbiology, Cherian Heart Foundation, International Centre for Cardio Thoracic and Vascular Diseases, Chennai, India.
Prabha MS, Verghese S., Endogenous retrovirus subtypes in pigs, Onl J Vet Res., 26 (11): 810-814, 2022. Porcine xenograft can transmit porcine endogenous γ-retrovirus from murine leukemia and feline leukemia viruses. We developed PCR for GAG gene region to detect porcine retrovirus in aortic and pulmonary valves and myocardium from xenografted tissue with kidney epithelial PK-15 cell line as positive PCR control. To distinguish retrovirus sequences, PCR for env A, env B and env C oligonuclotide primers were used. Of 50 PCR’s, retrovirus DNA was detected in 100% envA, 82% envB, both 44%, and envC 48%. Our results suggest that retroviral DNA is present in pig genome regardless of tissue. However, DNA retrovirus may differ from distribution of transcriptionally active retroviruses and may not reflect results of our analysis. Therefore, factors that influence retrovirus expression cannot eliminate retroviruses from pig xenotransplants. We find that common retroviruses could not determine which particles were functional but our results suggest that retroviral DNA is consistently present in porcine genome.
Key words: retroviruses, porcine, xenografts, zoonosis.