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Online Journal of Veterinary Research©


Volume 23 (8):782-785, 2019.


Effect of GnRH and serum estradiol on reproduction in buffaloes

with or without  retained fetal membranes.


Abdulamir Ali Alzahid1, Enas Ali Al–Yassiri2, Talib Mosa Alhamedawi2


Department(s) of Surgery and Obstetrics, College(s) of Veterinary Medicine. 1University of  Kerbala, 2University of  Baghdad, Iraq.




Ali Alzahid A, Ali Al–Yassiri E, Mosa Alhamedawi T., Effect of GnRH and serum estradiol on reproduction in buffaloes with or without retained fetal membranes, Onl J Vet Res., 23 (8):782-, 2019. Blood samples for estradiol were taken from twenty 3rd trimester pregnant buffalo 1-2 weeks before partus. After parturition, those with or without retained fetal membranes (RFM) were injected 0.021mg (5ml) Gonadotropic releasing hormone (GnRH) IM. We then monitored days to estrus, open days, service/conception and pregnancy rates. We found ~38% lower serum estradiol (22.8±4.64 pg/ml) in buffalo with RFM compared with those that subsequently expelled the fetal membranes (37.1+3.82 pg/ml; P < 0.01). Buffalo with RFM took ~42% (28.56±4.74 days:P < 0.01) longer to reach estrus compared with those without RFM. Our results suggested that to conceive, buffalo with retained membranes required ~29% more servicing by bulls (P < 0.01) than those without RFM. We also found ~26% more (P < 0.01) open days in those with RFM but no effect on pregnancy rates.


Key Words- Buffaloes, Retained fetal membrane, Estradiol, GnRH, reproduction.