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Online Journal of Veterinary Research

Volume 24 (3):166-174, 2020.

Synthesis and characterization of Juglans rejia resin aqueous extract silver nanoparticles and antibacterial effects.


Seyed Amin Lotfi1, Mehdi Fazeli1, Amin Reza Zolghadr2, Fatemeh Namazi3, Hossein Shahroki Sardo1


1Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, 2Department of Chemistry, Faculty of Science, Shiraz University, 3Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran




Lotfi SA, Fazeli M, Zolghadr AR, Namazi F, Sardo HS., Synthesis and characterization of Juglans rejia resin aqueous extract silver nanoparticles and antibacterial effects, Onl J Vet Res., 24 (3):166-174, 2020. We synthetized silver nanoparticles with Juglans rejia resin aqueous extract. The effect of different concentrations of silver nitrate and resin for production of nanoparticles was detected by spectrophotometery at λmax of 482 nm. Particles were monitored by emission scanning electron microscopy and X-ray diffraction. By electron scan imaging we found hexagonal shaped nanoparticles 25-35 nm suggesting reduction of silver ions by resin extract. By X-ray diffraction we found sharp Bragg peaks of elemental silver at 2◦ diffraction peaks at 38.1 ̊, 44.2 ̊, 64.4 ̊ and 77.4 ̊ in 2θ, matching Joint Committee on Powder Diffraction standard 040783. By disc diffusion, we determined effect of J. rejia resin, nanoparticles with resin and empty particle controls on bacteria. Particles with resin mildly inhibited E. coli, P. aeruginosa and S. aureus but not B. subtilis whereas J. rejia resin did not elicit any inhibition.


Keywords: Silver nanoparticles, Juglans rejia, antimicrobial.