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OJVRTM

Online Journal of Veterinary Research©

Volume 20(2):82-93, 2016.

 

Antimicrobial activity of probiotic Lactobacillus strains isolated from raw milk

 

Rasha Eid (DVM, PhD)1, Jakeen El Jakee (DVM, PhD)2, Amal Rashidy (DVM, PhD)1, Hanaa Asfour (DVM, PhD)1,

Shimaa omara (DVM)3, Zafar Mahmood (Msc, PhD)4, Jens Hahne (MSc, PhD)5, Ahmed Adel Seida (DVM, PhD)2*

 

1Animal Reproduction Research Institute, Giza, 2Department of Microbiology and Immunology, Faculty of Veterinary Medicine, Cairo University,  3Department of Microbiology and Immunology, National Research Center, Cairo, Egypt. 4Graduate School of Life Sciences, University of Wuerzburg, Würzburg, Germany, 5Institute of Cancer Research, London, England

 

ABSTRACT

 

Eid R, El Jakee J, Rashidy A, Asfour H, Omara S, Mahmood  Z, Hahne J, Seida AA., Antimicrobial activity of probiotic Lactobacillus strains isolated from raw milk, Onl J Vet Res., 20(2):82-93, 2016. Bacteriocins (BC) can inhibit mastitis pathogens S. aureus, E. coli, Y. enterocolitica, S. uberis and S. xylosus. BC producing Lactobacillus species from raw cow, buffalo, ewe and goat milk were isolated and purified by gel filtration. BC molecular weights (MW) varied between 3 -30 kDa, showed high thermal stability up to 100°C, and were active over a pH range of 3 to 10. Bacterial growth was inhibited by BC depending on pH, temperature and MW. For example, BC from L. acidophilus MW3.5 or 6.kDa from cows milk did not inhibit S. xylosus or Y. enterocolitica whereas MW4kDa did.  Similarly, BC from L pentosus MW17KdA from Buffalo milk did not inhibit S. uberis or Y enterocolitica but the MW9.8KdA did. BC’s of L. delbrueckii subspecies bulgaricus with MW16.5 and 21 KdA from cow or ewes inhibited all bacteria tested. BC of L. brevis MW4.6kDa from goat milk inhibited S. xylosus and E. coli and BC L. helveticus (4.3kDa) showed inhibition zone against S. aureus and E. coli.

 

KEY-WORDS: Milk, Lactobacillus species, Antimicrobial Activities, Bacteriocin.

 

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