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Online Journal of Veterinary Research

Volume 21(9):616-620, 2017


Hydroxyapatite stem cell repair of radial fracture gap in rabbits


Seifollah N Dehghani DVM MVSC1, Mahammad E Bahrololum MS PhD1, Abbas A Ghaderi DVM PhD2, Safura B Ghadirian DVM2 ,

Zahra Faghihi MD2, Mahdi Javidi MS PhD1 and Mohamad J Emami MD3.


Department of Surgery, School of Veterinary Medicine, and (1) Material Science, Collage of Engineering, Shiraz University, (2) Institute for Cancer Research and (3)Bone and joint disease research center, Shiraz University of Medical Science, Shiraz, Iran. Correspondence: Dehghani SN. Department of Surgery, School of Veterinary Medicine, Shiraz University, Shiraz, 71345-1731. Iran. Phone: 0098 9171184360. E mail:




Dehghani SN, Bahrololum ME, Ghaderi AA, Ghadirian SB, Faghihi Z, Javidi M, Emami MJ., Hydroxyapatite stem cell repair of radial fracture gap in rabbits, Onl J Vet Res., 21(9):616-, 2017. Nanosized hydroxyapatite is used to replace bone gaps due to its similarity with the mineral constituents of bone. The effect of hydroxyapatite with or without stem cells implants on repair of radial bone gap fracture in groups of 5 New Zealand white rabbits is described. Under anaesthesia, a 2cm section of right forelimb radial bone was removed by osteoma. Bone gaps were filled with a 1ml solution of ~2M adipose tissue derived stem cells, or a cylinder shaped hydroxyapatite pellet, or a 1ml solution of ~2M adipose tissue derived stem cells injected around the hydroxyapatite pellet implant. Controls were operated, but not treated or normal. Limbs were evaluated radiographically every 21 days for 3 months. At 3 months, rabbits were euthanized and bones subjected to biomechanical tests. Bone formation Lane and Sandhu radiological scores at 21 days were 1.4 0.4 for stem cell 2.5 1.00 pellet, 2.00 0.1 pellet with stem cell implants and 2.00 0.2 for controls. By day 42, scores were 2.2 0.84, 3.5 0.75, 4.00 0.3 and 4.00 0.25 for the same measurements, respectively. By day 63 there were no significant differences in scores between stem cell, pellet or normal bone, but with controls there was (P < 0.005). Results suggest that stem cells may accelerate bone healing alone or with hydroxyapatite.


Key words: Hydroxyapatite, stem cell, bone repair, biomechanics, radiological evaluation.