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OJVRTM

Online Journal of Veterinary Research©

Volume 19(6): 436-445, 2015.


Osteogenic potential of subcutaneous adipose-derived stem cells in a rabbit model

 

Shaterzadeh- Yazdi H1, Mehrabani D2, Khodakaram- Tafti A1, Dianatpour M2, Zare SH2, Tamaddon A2, Razeghian- Jahromi I1

 

1School of Veterinary Medicine, Shiraz University, 2Shiraz University of Medical Sciences, Shiraz, Iran

 

Abstract

 

Shaterzadeh Yazdi H, Mehrabani D, Khodakaram Tafti A, Dianatpour M, Zare SH, Tamaddon A, Razeghian Jahromi I., Osteogenic potential of subcutaneous adipose-derived stem cells in a rabbit model, Onl J Vet Res., 19(6): 436-445, 2015. Subcutaneous adipose-derived stem cells (ADSCs) can be provided in large quantities as an alternative source in regenerative medicine. We report osteogenic potential of these cells in rabbits. Four gram subcutaneous adipose tissue samples from anaesthetized adult Dutch white rabbits were digested in collagenase type II and cultured in DMEM. Cells were enumerated up to the 4th passage and population doubling time (PDT) was determined. Karyotyping and RT-PCR were used to evaluate normal diploid cells and expression of ADSCs or hematopoietic stem cell markers including CD73, CD34 and CD45. Adipogenic and osteogenic potential of differentiated cells was confirmed by Oil Red O and Alizarin Red S staining. Isolated cells had fibroblastic like morphology with an S shaped growth and a PDT of 70.2 hours. Until the 4th passage cells had normal a karyotype. RT- PCR showed that the cells were CD73 positive but CD34 and CD45 negative. With special staining, evidence of calcium deposition and formation of fat cells confirmed osteogenic and adipogenic properties. These findings suggest osteogenic potential of subcutaneous ADSCs for application in regenerative medicine and tissue engineering.

 

Key words: osteogenic potential, regenerative medicine, karyotype and RT-PCR analysis, ADSCs, rabbit.


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