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OJVRTM
Online
Journal of Veterinary Research©
Volume 19(6): 436-445, 2015.
Osteogenic
potential of subcutaneous adipose-derived stem cells in a rabbit model
Shaterzadeh- Yazdi H1,
Mehrabani D2, Khodakaram-
Tafti A1, Dianatpour
M2, Zare SH2, Tamaddon A2, Razeghian-
Jahromi I1
1School of Veterinary Medicine, Shiraz
University, 2Shiraz University of Medical Sciences, Shiraz, Iran
Abstract
Shaterzadeh Yazdi H, Mehrabani
D, Khodakaram Tafti A, Dianatpour M, Zare SH, Tamaddon A, Razeghian Jahromi I., Osteogenic potential of subcutaneous
adipose-derived stem cells in a rabbit model, Onl J
Vet Res., 19(6): 436-445, 2015. Subcutaneous adipose-derived stem cells
(ADSCs) can be provided in large quantities as an alternative source in
regenerative medicine. We report osteogenic potential of these cells in
rabbits. Four gram subcutaneous adipose tissue samples from anaesthetized adult
Dutch white rabbits were digested in collagenase type II and cultured in DMEM.
Cells were enumerated up to the 4th passage and population doubling
time (PDT) was determined. Karyotyping and RT-PCR were used to evaluate normal
diploid cells and expression of ADSCs or hematopoietic stem cell markers
including CD73, CD34 and CD45. Adipogenic and
osteogenic potential of differentiated cells was confirmed by Oil Red O and
Alizarin Red S staining. Isolated cells had fibroblastic like morphology
with an S shaped growth and a PDT of 70.2 hours. Until the 4th
passage cells had normal a karyotype. RT- PCR showed that the cells were CD73
positive but CD34 and CD45 negative. With special staining, evidence of calcium
deposition and formation of fat cells confirmed osteogenic and adipogenic properties. These findings suggest osteogenic
potential of subcutaneous ADSCs for application in regenerative medicine and
tissue engineering.
Key words: osteogenic potential,
regenerative medicine, karyotype and RT-PCR analysis, ADSCs, rabbit.
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