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OJVRTM

Online Journal of Veterinary Research

(including medical and laboratory Research)

Established 1994

ISSN 1328-925X

 

Volume 22 (10):933-940, 2018.


Production of pepsin by bacteria (Staphylococcus sciuri) and fungi (Pythium sp)

from soil and rumens cultured in waste whey and brans.

 

Omer Dhiaa Al-Deen Salah, Dhafer Fakhri Al-Rawi, Harith Kamil Buniya.

 

College of Education, University of Anbar, Iraq.

 

ABSTRACT

 

All-Deen Salah OD, Al-Rawi DF, BuniyaHK., Production of pepsin by bacteria (Staphylococcus sciuri) and fungi (Pythium sp) from soil and rumens cultured in waste whey and brans, Onl J Vet Res., 22 (10):933-940, 2018. Pepsin producing bacteria and fungi from 10 soil and goat, sheep and cattle rumen content samples were identified by Biomerieux VITEK 2d and standard methods, then mixed with pasteurized whey or brans to determine optimal pepsin production. Our results suggested that Staphylococcus sciuri and Pythium sp produced optimal clotting at ~15 min at 50C˚, pH 2.0 in 10% whey with 1.0 ml inoculum incubated 24h yielding 4.6 units/ml pepsin.

 

Keyword : Pepsin production, Soil, Rumen, Staphylococcus sciuri, Pythium sp, whey, brans.


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