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Online Journal of Veterinary Research©
Volume 25(2): 131-142, 2021.
Effect of Pyocyanin from Pseudomonas aeruginosa on Aspergillus fumigatus
from patients and sheep with chronic respiratory infection
Riyadh Ajmi Salman and Zainab AA Al-Haddad.
Zoonosis diseases unit, College of Veterinary Medicine, University of Baghdad, Iraq.
Salman RA, Al-haddad ZA., Effect of Pyocyanin from Pseudomonas aeruginosa on Aspergillus fumigatus from patients and sheep with chronic respiratory infection, Onl J Vet Res., 25(2): 131 -142, 2021. We report effect of pyocyanin extracted from Pseudomonas aeruginosa on growth of Aspergillus fumigatus isolated from sputum and nasal discharges of 100 patients and sheep with chronic respiratory infection, determined by nasal discharge, dyspnea and coughing. We isolated P. aeruginosa from 4 patients and 5 sheep and A. fumigatus from 12 subjects and 2 sheep. Pyocyanin was extracted from P. aeruginosa grown on king’s B broth to yield 3000µg/ml. We tested 250 to 6000 µg/ml pyocyanin on growth of A. fumigatus on sabouraud dextrose agar after 7 days of 25ᵒc incubation with 250 µg/ml inhibiting ~37% 500 ~50%, 1000 ~63%, 2000 81%, 3000 87 4000 95% and 6000 µg/ml 100%. We find that pyocyanin from P. aeruginosa inhibits A. fumigatus from respiratory infections in vitro in a dose dependent manner.
Key words: Pyocyanin, P. aeruginosa, A. fuminatus isolates, human, sheep.