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Online Journal of Veterinary Research©
Volume (10):942-955, 2019.
Effect of Helicobacter pylori antigens on hypersensitive footpad test,
interleukin-6 and gamma immunoglobin in male Albino rats.
Samir SH Al-Jabir, Ikram AA Al-Samarraae.
Microbiology, Faculty of Veterinary Medicine, University of Baghdad, Baghdad, Iraq.
Al-Jabir SSH, Al-Samarraae I., Effect of Helicobacter pylori antigens on hypersensitive footpad test, interleukin-6 and gamma immunoglobin in male Albino rats, Onl J Vet Res., (10):942-955, 2019. Groups of 6 albino male rats each were injected subcutaneous (SC) 0.5 or 1 mg/ml killed whole cell Helicobacter pylori sonicated antigen or 1x109 cell/ml non-sonicated antigen. A booster shoot was given at 14 days. Controls were injected 1ml PBS. Blood was taken at 12, 28, 42 and 56 days for serum interleukin-6 and gamma immunoglobin. After 18 days, a delayed hypersensitivity reaction skin (DHL) test of 0.2ml sonicated antigen was done at left footpad and 0.2ml PBS (pH 7.2) controls in the right pad and skin thickness (mm) measured at 24, 48 and 72 hours. Compared with left pad PBS controls, we found reduced (P < 0.05) DHL but only after 48h with 0.5mg/ml (~-18%) or 1x109 cell/ml (~-13%). There was no difference with higher dose 1mg/ml antigen. Compared with before test controls pad size increased 12-16% as well as in those given only PBS. In contrast, interleukin-6 levels increased ~21-40% in rats given the higher dose (P<0.05). Similarly we found large increases (P < 0.05) in gamma-immunoglobulin throughout (48-66%). Our results suggest that the lower dose sonicated antigen may produce a greater skin reaction but the higher dose was more immuno-stimulatory.
Key Words: Helicobacter, antigens, immunity, rat. Raw data provided.