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OJVRTM
Online
Journal of Veterinary Research©
Volume 25 (5): 327-330,
2021.
In silico
interactions between DNA and platelet P2Y1 receptor.
Arnab
Mukherjee
Indian Institute of Technology, Madras, India.
ABSTRACT
Mukherjee A., In silico interactions between DNA and platelet p2y1
receptor, Onl J Vet Res., 25 (5): 327-330, 2021. Purinergic receptors are proteins
that respond to sensitization by purine nucleotides, setting off signal
transduction pathways eliciting ligand-gated ion channels
P2X and G-Protein Coupled Receptors P2Y type responses. A specific purine-receptor is expressed
greatly on platelet surface as a high affinity ADP receptor to induce platelet aggregation.
In
silico steric force analysis was
done to determine if P2Y1 receptor which
evinces a high affinity for ADP could bind
DNA as well. Secondary structure of protein proximal
to docking site almost fitted into minor groove helix required for protein DNA
interactions. Amino acids found close to docking included ASP that bonds
hydrogen with multiple bases, ASN adenosine as donor and acceptor as P2Y1 is a
high affinity ADP receptor, SER bidentate H with guanine, ARG favors H-bond and
PHE, PRO and ILE enhance Van der Waals forces by alkyl side chain and flat
surface aromatic rings. Author surmises that In silico docking yielded stable and steric DNA-P2Y1 complexes with
~>70% due to Van Der Waal forces. Docking site analysis revealed amino acids
that could favor DNA-protein interactions suggesting that high affinity ADP receptorP2Y1 might interact
with double stranded DNA and terminal helices.
KEYWORDS: platelets, purinergic receptors,
P2Y1, DNA binding proteins, ADP, docking.
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