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OJVRTM
Online Journal of Veterinary Research©
Volume 18(4): 331-338, 2014. Redacted 2017.
Analysis of ellagic acid
in Punica granatum
flower extract by high performance liquid chromatography
Khatereh Safavi Naeini1, Hamid Rajaiyan2, Saeed Nazifi3*
1,2Department(s) of Pharmacology and 3Clinical Pathology, School of Veterinary Medicine,
Shiraz University, Shiraz, Iran
ABSTRACT
Naeini KS, Rajaiyan
H, Nazifi S., Analysis of ellagic
acid in Punica granatum
flower extract using high performance liquid chromatography, Onl J Vet Res., 18(4): 331-338, 2014.. Punica
granatum flower (PGFL), apparently may have
medicinal properties. We measured ellagic acid in hydroalcoholic extract of PGFL, by high performance liquid
chromatography (HPLC) using Inertsil C18 (250 x
4.6) mm, 5μ column at wavelength 254 nm with a retention time (RT) of 38.2
minutes. The linear regression analysis data for the calibration plots showed a
linear relationship r2 = 0.9987. According to the International
Conference on Harmonization (ICH) guidelines, the method was validated in terms
of its linearity, specificity, recovery, precision, robustness and solution
stability.
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