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OJVRTM
Online Journal of Veterinary Research©
Volume
18(7): 587-595, 2014. Redacted 2017..
Effect of quaternary ammonium compounds and temperature on shearing
utensils
contaminated by Corynebacterium pseudotuberculosis.
Estevao Belchior, Silvia Graciela (PhD), Alvarez, Laura Alejandra(BSc).
Centro
Regional de Investigación y Desarrollo Científico-Tecnológico (CRIDECIT).
Facultad de Ciencias Naturales. Universidad Nacional de la Patagonia San Juan
Bosco. Ciudad Universitaria Km. 4. Comodoro Rivadavia. C.P. (9000). Chubut.
Argentina.
ABSTRACT
Belchior E, Graciela S,
Laura Alejandra A., Effect of quaternary ammonium compounds and temperature on
shearing utensils contaminated by Corynebacterium pseudotuberculosis, Onl J Vet Res., 18(7): 587-595,
2014. Prevalence of Corynebacterium
pseudotuberculosis, causal agent of sheep caseous lymphadenitis (CLA) in Southern Argentina, reaches
70%. C. pseudotuberculosis survive for
long periods on infected premises and soils. Susceptibility of C. pseudotuberculosis to treatment with quaternary
ammonium compounds (QA) and exposure to 40, 50 and 65°C for 120 min on clean and with
contaminants (interfering substances such as soil), shearing clippers, cutters and combs is described. C. pseudotuberculosis was eliminated by 30s exposure
to 1:200 dilution and 10m exposure to 1:104 dilutions QA. At 1:200
dilution QA with interfering substances bacteria were inactivated at 60s
exposure. Without contaminants on utensils, C
pseudotuberculosis decreased slightly for 40 min at 40 °C (6.67 to 5.95 log),
but surviving bacteria
remained constant. At 50°C bacteria decreased 60 min (6.67 log to 4.55 log) and stayed at average values. At 65°C
bacteria were inactivated within 3 min of contact but in the presence of contaminants
required 5 min. Utensil tests showed than the 1:200 dilution of the
disinfectant was effective in eliminating C.
pseudotuberculosis from the surface of the cutter and comb
sides of the shearing clippers after 1 min of contact time. With
the 1:104 dilution, the shearing
clippers remained
contaminated for 20 min. At 65 °C, the bacterial population was completely
inactivated within 3 min of contact time. At lower temperatures, the treatment
was ineffective. Results suggest that Quaternary ammonium compounds are effective at dilutions of 1:2000 with or
without contaminants for about 1 min whereas exposure of utensils to 65°C with contaminant decreases
bacteria 90% in 56 sec
KEY WORDS: Caseous
lymphadenitis; prevention and control; Patagonia Argentina.
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