Volume 23 (1):102-111, 2019.
Effect of autologous platelet rich plasma on full thickness skin graft healing in rams.
Saddam Khalid Humadi1, Hiba Abdulaziz Shekho1, Ahmed Abdullah Sultan2
1Department(s) of Surgery, 2Pathology, College of Veterinary Medicine, Tikrit University. Iraq.
Humadi SK, Shekho HA, Sultan AA., Effect of autologous platelet rich plasma on full thickness skin graft healing in rams. Onl J Vet Res., 23 (1):102-111, 2019. Authors report effect of injections of autologous platelet rich plasma (PRP) on macroscopic and microscopic healing of skin grafts in 12 rams over 16 days. Under sedation and local anesthesia, ~2cm2 thick skin flaps were excised from a right dorsal site replaced with a left site of the same ram. Grafts were fixed by simple interrupted sutured 2.0 silk. Right site grafts (n=12) were subcutaneously injected with PRP and left side controls grafted and sutured without PRP (n=12). Grafts were protected with sterile gauze and adhesive tape. Graft healing was scored 0-4 excellent to poor every 4 days for 21 days. Grafts with PRP, healed homogenously with adjacent skin with exception of 2 with discolored dry wounds. However, in non-treated control grafts we found 8 instances of non-homogeneous dry wound. In controls, we scored grafting 2 by day, and 1.5 in those given PRP. By day 8 all grafts treated with PRP (score 0) healed whereas in controls, only by day 16. Microscopy of grafts with PRP revealed earlier proliferation of fibroblasts with increased extracellular collagen matrix, re-epithelialization, regeneration of hair follicles, sebaceous and sweat glands. Initially there were greater number of polymorphonuclear cells in non-treated grafts. Findings suggest that PRP may enhance wound healing of grafts in rams.
Key words: Skin graft, Platelets Rich Plasma, Sheep.