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 Online Journal of Veterinary Research

Volume 22 (6):518-525, 2018.

Systematic review and meta-analysis protocol for Fasciola DNA vaccines.


Rama Jayaraj MVetSc PhD MPH1*, Chellan Kumarasamy BTech2, David Piedrafita PhD3, Peter Smooker PhD4.


1Charles Darwin University, Darwin, Northern Territory 0909, 2University of Adelaide, Adelaide, 3Faculty of Science and Technology Federation University, Churchill, NZ, 4School of Science, RMIT University, Bundoora West Campus, Vic, Australia. *Rama.Jayaraj@cdu.edu.au




Jayaraj R, Kumarasamy C, Piedrafita D, Smooker P., Systematic review and meta-analysis protocol for Fasciola DNA vaccines, Onl J Vet Res., 22 (6):518-525, 2018. We describe a systematic review and meta-analysis of immune response and protective efficacy of Fasciola DNA vaccines following PRISMA-P (2015) guidelines (Moher et al. 2015). Multiple databases of published articles were independently assessed for eligibility and meta-analysis to determine protection rate estimates and pooled odds Ratio with 95% confidence intervals. Heterogeneity was estimated by Higgins I2 statistic and publication bias via funnel plots. A narrative synthesis of systematic review was done where meta-analysis was not possible. Previous studies evaluated effectiveness of DNA encoding Fasciola vaccine candidates, but results are highly variable due to heterogeneity in vaccines and their protocols. We attempt to elucidate this observed heterogeneity for development of DNA vaccines against fascioliasis.


Key Words: Fasciola hepatica, Fasciola gigantica, systematic review, meta-analysis, DNA vaccines, protective efficacy.