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OJVRTM

 

Online Journal of Veterinary Research©

 

Volume 10 (2):134-144, 2006. Extensively redacted 2018..


Antimicrobial probiotic effects of selected avian intestinal lactic acid bacteria. 

 

Mojgani Na*, Torshizi Mb, Molae Mc

 

aDepartment of Biotechnology, Razi Vaccine and Serum Research Institute, c bDepartment of Animal Science, College of Agriculture, Tarbiat Modarres University, IR Iran

 

SUMMARY

 

Mojgani N, Torshizi M, Molae M., Probiotic potential of Avian Intestinal Lactic Acid Bacteria  Onl J Vet Res., 10 (2):134-145, 2006. A total of 250 chicken intestinal specimens were screened for Lactic Acid Bacteria (LAB) by routine cultural, morphological and biochemical reactions. Seventy five were identified and characterized for probiotic activity. Lactobacillus acidophilus RNL26, Lactobacillus fermentum RNL 44 and Lactobacillus salivarrius RNL 49T were resistant to acidic pH and high bile salt concentrations. The strains inhibited gram positive Clostridium perfringens, Staphylococcus aureus and Listeria monocytogenes. Only L. fermentum RNL 44 inhibited gram negative Salmonella enteritidis and S. typhimurium. Because neutralized and catalase treated supernatant fluid from L. acidophilus RNL26 and L. salivarrius RNL 49 retained antibacterial activity, we surmise that bacteriocin was responsible for antimicrobial effects.  We conclude that although the strains of LAB survived acidic conditions and bile concentration to variable extent, only L. acidophilus RNL 26, L. fermentum RNL 44 and L. salivarrius RNL 49 could be considered as potential ingredients for a chicken probiotic feed formulation. Results of our In vitro study with LAB strains should be confirmed In vivo. The strains were characterized in vitro for cholesterol removal, aggregation, co-aggregation, and cell surface hydrophobicity.

 

Key Words: Lactic Acid Bacteria, In vitro, Antimicrobial, Poultry, Probiotic.


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