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Online Journal of Veterinary Research©
Volume 24 (1):60-65, 2020.
Periodontal histopathology induced by tooth ligature of Porphyromonas gingivalis and Fusobacterium nucleatum in rats.
Liew Nyan Fatt (DDS)1, Ee Gee Yong (DDS)1, Leong Xin Fang (BSc, PhD)1, Nuramirah Azizan (BSc, MSc)1, Badiah Baharin (DDS, MClinDent)2,
Nurulhuda Mohd (DDS, MClinDent)2, Shaqinah Nurrul Nasruddin (DVM, PhD)1*
1Department of Craniofacial Diagnostics and Biosciences, Faculty of Dentistry, Universiti Kebangsaan Malaysia and 2Department of Restorative Dentistry, Faculty of Dentistry, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia. *Corresponding author: Shaqinah Nurrul Nasruddin, Department of Craniofacial Diagnostics and Biosciences, Faculty of Dentistry, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
Fatt LN., Gee Yong E, Xin Fang L, Azizan N, Baharin B, Mohd N, Nasruddin SN., Periodontal histopathology induced by tooth ligature of Porphyromonas gingivalis and Fusobacterium nucleatum in rats, Onl J Vet Res., 24 (1):60-65, 2020. Dental histopathological changes in groups of 6 adult female Sprague Dawley rats each infected by Porphyromonas gingivalis with or without Fusobacterium nucleatum are described. Ligation technique in rats can induce osteoclastogenesis, bone loss and irritate gingival tissues to produce plaque and immune responses that cause periodontitis as described by Oz & Puleo, (2011). Ligatures in “8” with 4/0 non-resorbable sterile silk thread were placed around inferior lower incisors embedded with the bacteria to induce plaque and development of periodontitis. We differ in that usually molar teeth are ligated, not lower incisors. Infection was maintained by oral inoculation of 108 cfu/ml bacteria 3 days a week for 4 weeks as described by Kesavalu et al., (2007). Controls were inoculated 1ml sterile saline without ligation. After 4 weeks rats were euthanized and heads decalcified for 12-hand fixed in formalin for microscopy. Lesions were scored 0% affected (-) and 30 to 60% (+ to +++). Moderate gingival hyperplasia with uneven thickness of epithelium and moderate to severe congestion (++) was found particularly at the periodontal ligament and gingival connective tissue area. We did not observe any obvious differences in rats infected with one or both bacteria. Our results differ from those using molar ligation technique. As expected, controls had no lesions.
Keywords: periodontitis, rat, bacteria, oral inoculation, Raw data Provided.