2022-2032 All Rights Reserved. Online Journal of Veterinary Research. You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to OJVR. This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editors. This journal satisfies the refereeing requirements (DEST) for the Higher Education Research Data Collection (Australia). Linking: To link to this page or any pages linking to this page you must link directly to this page only here rather than put up your own page.


Online Journal of Veterinary Research


Volume 26 (7): 537-545, 2022.

Conjugated polyclonal antibody mouse IgG3 subclass in rabbit.


Somayeh Dadashi, Jafar Majidi, Leili Aghebati Maleki, Jalal Abdolalizadeh, Behzad Baradaran, Zahra Moradi,

Sadeq Eivazi, Majid Ahmadi, Naeemeh Majidi


Tabriz University of Medical Sciences, Tabriz, Iran.




Dadashi S, Majidi J, Maleki LA, Abdolalizadeh J, Baradaran B, Moradi Z, Eivazi S, Ahmadi M, Majidi N., Conjugated polyclonal antibody mouse IgG3 subclass in rabbit, Onl J Vet Res., Volume 26 (7): 537-545, 2022. Monoclonal and polyclonal antibodies are used for indirect flow cytometric, ELISA and cytotoxicity assays. We report a polyclonal antibody against mouse IgG3 in rabbit. IgG3 was purified by sepharose beads conjugated with protein for affinity chromatography and its purity confirmed by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE). We then injected New Zealand white rabbit with the IgG3 intramuscularly and intradermally. We further purified antibody in rabbit serum with ammonium sulfate precipitation and ion-exchange chromatography and SDS-PAGE. This antibody was conjugated with horse radish peroxidase for titer of rabbit anti-mouse IgG3 and HRP of 64000 determined by ELISA. Purity reached ~95% and optimum dilution ~1:10000.


Key words: Anti-IgG3, Chromatography, Mouse IgG3, Polyclonal Antibody, Protein A.