MAinMAIN
©1996-2016 All Rights
Reserved. Online Journal of Veterinary Research . You may not store these pages
in any form except for your own personal use. All other usage or distribution
is illegal under international copyright treaties. Permission to use any of
these pages in any other way besides the before mentioned must be gained in
writing from the publisher. This article is exclusively copyrighted in its
entirety to OJVR. This article may be copied once but may not be, reproduced or
re-transmitted without the express permission of the editors. This journal
satisfies the refereeing requirements (DEST) for the Higher Education Research
Data Collection. Linking:To link to this page or any pages linking to this page
you must link directly to this page only here rather than put up your own page.
OJVRTM
Online Journal of Veterinary Research©
Volume 19(8): 486-490, 2015.
Polyclonal
antibody from rabbit for detection of bursal disease virus
Amir Shabdini Pashaki1, Mehdi Aminafshar1*, Mohammadreza
Safarnejad2, Seyyed Davood
Hoseini3
1Department
of Animal Science, Faculty of Agriculture and Natural Resources, Tehran Science
and Research Branch, Islamic Azad University, Tehran, Iran. 2Department
of Plant Viruses, Iranian Research Institute of Plant Protection, Tehran, Iran.
3Razi Vaccine and Serum Research Institute, Arak,
Iran.
Pashaki AS, Aminafshar M, Safarnejad M, Hoseini SD.,
Polyclonal antibody from rabbit for detection of bursal disease virus, Onl J Vet Res., 19(8): 486-490,
2015. Antibody for detection of Bursal disease (BD) induced in rabbits is
described. Killed BD viruses were emulsified with oil for inoculation in
rabbits and serum was then titrated after each of 4 inoculations. Antibody in
blood was purified by affinity chromatography using a protein A column. Alkaline phosphatase was conjugated to the
immunoglobulin which was then detected by double antibody sandwich
enzyme-linked immunosorbent (DAS-ELISA) and dot immunosorbent assays (DIBA).
These preliminary results suggest that the purified antibody could be used for
detection of infectious bursal disease virus.
Keywords: ELISA, Infectious Bursal Disease (IBD), DIBA, Polyclonal
Antibody.
nMAIN
FULL-TEXT (SUBSCRIPTION
OR PURCHASE TITLE $25USD)