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OJVRTM
Online Journal of
Veterinary Research©
Volume 17(11):600-607, 2013. Redacted 2017.
Phylogenetic
analysis of neuraminidase amino acid sequences of avian influenza virus H9N2
serotype isolates in Iran
Maryam Daghari1, Masoudreza Seyfi Abad Shapouri1*
and Mansour Mayahi2
1Departments
of Pathobiology and 2Clinical Sciences, School of Veterinary
Medicine, Shahid Chamran University,
Ahvaz, Iran
ABSTRACT
Daghari M, Shapouri
MSA, Mayahi M., Phylogenetic analysis of
neuraminidase amino acid sequences of avian influenza virus H9N2 serotype
isolates, Onl J Vet Res., 17(11):600-607,
2013. H9N2 serotype of
avian influenza A virus was first identified in Iran,
in 1998. To control losses a killed vaccine has been used. Genetic changes in the neuraminidase gene in
H9N2 isolates found in Iran from 1998 to 2011 are
described. Amino acid sequences of 24
isolates of H9N2 extracted from genebank
and 3 sequences were aligned to construct a phylogenetic tree using the
Neighbourhood Joining method. The viruses of a subgroup isolated
from 1998 to 2006 were found to be more similar to the prototype of the G1-like
group with 92.7- 96.3% similarity. In contrast, only 90.4 to 92.3% similarity was found in
another subgroup from 2006 to 2011 with the isolate HK/G1/97. This suggests
that these isolates may have changed over the years following the virus
entrance in 1998. There were no deletions in the neuraminidase stalk but the change in amino acid sequence of the
hem-adsorption sites in recent years could justify a classification
of 2 phylogenetic subgroups. Regular
monitoring of field isolates for vaccine production is recommended
Key Words: Avian influenza virus, H9N2, Neuraminidase
sequence, Phylogeny, Iran.
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