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OJVRTM

 

Online Journal of Veterinary Research©

(Including Medical and Laboratory Research)

Established 1994

ISSN 1328-925X

 

Volume 28 (9): 546-557, 2024.

Pectin lyase genes in fungal strains

 

Amit Kumar Dubey MSc, Sangeeta Yadav PhD, Gautam Anand MSc, Naveen C Bisht MSc, Dinesh Yadav PhD.

 

1Department of Biotechnology, Gorakhpur University, National Institute of Plant Genome Research, New Delhi,India.

 

ABSTRACT

 

Dubey AK, Yadav S, Anand G, Bisht NC, Yadav D., Pectin lyase genes in fungal strains, Onl J Vet Res., 28 (9): 546-557, 2024. Pectin lyase genes from Aspergillus niger MTCC404, A. versicolor MTCC3071, A. foetidus MTCC151, A. terricola MTCC7588, A. aculeatus MTCC7590, A. ficcum MTCC7591,  A. flavus MTCC7589 and Penicillium citrinum MTCC8897 were PCR amplified , gel eluted and sequenced. We found 18 pectin lyase gene sequences assigned GenBank JF447757-JF447774 from 396 to 940 bp subjected to in silico homology search, multiple sequence alignment, phylogenetic tree construction and motif analysis. BLAST identified pectin lyase gene family and multiple sequence alignment revealed highly conserved nucleotides G at position 259, G at 434 and C at 451. Phylogenetic tree generated 2 major clusters with pectin lyase genes amplified by same set of primers grouped irrespective of source organisms. Frequently motifs were Pec_Lyase_C superfamily, a typical feature of pectin lyase and pectate lyases.

 

Key words: Pectin lyase, PCR, multiple sequence alignment, motif, BLAST, Fungi.

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