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OJVRTM
Online Journal of Veterinary
Research©
Volume 23 (8):799-807, 2019.
Molecular
characterization of Escherichia coli types from
diarrheic and healthy dogs.
Ghanbarpour Reza1 DVM PhD,
Akhtardanesh Baharak2 DVM PhD, Afsahi Elham2 DVM, Sookhtanloo
Somayeh1 (DVM).
1Microbiology, 2Small
Animal Internal Medicine Departments of Faculty of Veterinary Medicine, Shahid Bahonar University, 76169-14111, Kerman, Iran
ABSTRACT
Reza G, Baharak A, Elham A, Somayeh S., Molecular characterization of Escherichia
coli strains from diarrheic and healthy dogs. Onl J
Vet Res., 23
(8):799-807, 2019. Escherichia coli (E. coli) strains are commensal inhabitants of
the gastrointestinal tract of dogs. However, a variety of E. coli types
have been associated with diarrheal illness in dogs. E. coli from fecal
samples of 65 dogs with acute diarrhea and 104 non-diarrheic ones were typed
and a phylogenetic tree was constructed. We found phylogenetic groups: A
(56.80%), B1 (13.06%), B2 912.42%) and D (17.15%) with 32.54% having at least
one virulence gene and 65.08% without any. Necro-toxic
E. coli were most prevalent with 26.15% diarrheic and 21.15% healthy dogs
positive for cnf1 gene, respectively. stx1
gene was found in 2 isolates (1.18%) from diarrheic and healthy dogs, whereas stx2
gene in 2 from diarrheic dogs. We detected 12 (7.10%) eae
enteropathogenic E
Coli strains 10.8% from diarrheic and 4.80% from healthy dogs. None of the
isolates had ST1, LT1, cnf2 or ipaH
genetic markers. We find similarities in phylogenetic distribution of E.
coli isolates from diarrheic and healthy dogs; although there are
differences in presence and prevalence of
virulence genes between both groups.
Key
words: Escherichia coli; pathotype, diarrhea, dog.
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