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OJVRTM

Online Journal of Veterinary Research

 

Volume 23 (8):799-807, 2019.


Molecular characterization of Escherichia coli types from diarrheic and healthy dogs.

 

Ghanbarpour Reza1 DVM PhD, Akhtardanesh Baharak2 DVM PhD, Afsahi Elham2 DVM, Sookhtanloo Somayeh1 (DVM).

 

1Microbiology, 2Small Animal Internal Medicine Departments of Faculty of Veterinary Medicine, Shahid Bahonar University, 76169-14111, Kerman, Iran

 

ABSTRACT

 

Reza G, Baharak A, Elham A, Somayeh S., Molecular characterization of Escherichia coli strains from diarrheic and healthy dogs. Onl J Vet Res., 23 (8):799-807, 2019. Escherichia coli (E. coli) strains are commensal inhabitants of the gastrointestinal tract of dogs. However, a variety of E. coli types have been associated with diarrheal illness in dogs. E. coli from fecal samples of 65 dogs with acute diarrhea and 104 non-diarrheic ones were typed and a phylogenetic tree was constructed. We found phylogenetic groups: A (56.80%), B1 (13.06%), B2 912.42%) and D (17.15%) with 32.54% having at least one virulence gene and 65.08% without any. Necro-toxic E. coli were most prevalent with 26.15% diarrheic and 21.15% healthy dogs positive for cnf1 gene, respectively. stx1 gene was found in 2 isolates (1.18%) from diarrheic and healthy dogs, whereas stx2 gene in 2 from diarrheic dogs. We detected 12 (7.10%) eae enteropathogenic E Coli strains 10.8% from diarrheic and 4.80% from healthy dogs. None of the isolates had ST1, LT1, cnf2 or ipaH genetic markers. We find similarities in phylogenetic distribution of E. coli isolates from diarrheic and healthy dogs; although there are differences in presence and prevalence of virulence genes between both groups.

 

Key words: Escherichia coli; pathotype, diarrhea, dog.


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