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OJVRTM
Online Journal of Veterinary Research©
Volume 17 (12):675-684, 2013. Extensively Redacted
2017.
Molecular analysis of virulence factors of Pasteurella multocida from sheep and goats
Sameneh Daneshlari1, Yahya Tahamtan2*, Mohammad Kargar1
1Departments of Microbiology, Jahrom
Branch, Islamic Azad University, Jahrom and 2Razi
Vaccine and Serum Research Institute-Shiraz, Iran
ABSTRACT
Daneshlari
S, Tahamtan Y, Kargar M.,
Molecular analysis of virulence factors of Pasteurella
multocida from sheep and goats, Onl
J Vet Res., 17 (12):675-684, 2013. Thirty one
isolates of Pasteurella multocida
from 500 cases of clinical respiratory disease in sheep and goats were
subjected to PCR assay. All isolates
were serogroup A P. multocida except for 2
serogroup D. P. multocida
was the most frequent pathogenic isolate recovered. Isolates were
characterized by 5 virulence factor genes. PCR showed that outer membrane
proteins (omph) occurred in most small
ruminant strains. Virulent factor genes pfhA, hgbA, and toxA,
were present in 7% of strains and exhibited distinctive associations with
serogroup A. However toxA VF was not found in
serogroup D. These could provide
possible vaccine candidates against P. multocida serotypes.
Keywords”
Pasteurella multocida,
virulence, PCR, respiratory infections, Sheep, Goat
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