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Online Journal of Veterinary Research©
(Including Medical and Laboratory Research)
Volume 25 (9):666-671, 2021.
Incidence of canine parvovirus by hemagglutination, hemagglutination inhibition and PCR tests.
S Parthiban, HK Mukhopadhyay, K Ravikumar, PX Antony and RM Pillai
Department of Veterinary Microbiology, Rajiv Gandhi College of Veterinary and Animal Sciences, Puducherry-605 009.
Parthiban S, Mukhopadhyay HK, Ravikumar K, Antony PX, Pillai RM., Incidence of canine parvovirus by hemagglutination, hemagglutination inhibition and PCR tests, Onl J Vet Res., 25 (9):666-671, 2021. One hundred twenty eight fecal and rectal samples were taken from dogs with diarrhea and/or enteritis. The samples were clarified, processed and subjected to hemagglutination (HA), hemagglutination inhibition (HI) and polymerase chain reaction (PCR) for canine parvovirus (CPV). By HA, 47/128 (36.7%) samples had titers 1:32 and above positive for CPV but 81 (63.29%) had weak positive titers below 1:32. Highly positive CPV titers were 1:64 and above whereas weak samples <1:32. Of 47 positive samples, 36 were highly positive and 11 weak positive. By hemagglutination inhibition test (HI) with CPV hyper immune rabbit serum all 47 were inhibited. By PCR we found 47 (36.7%) positive for CPV infections by HA assay confirmed by hem-agglutination inhibition tests. 68 (53.1%) samples were found to be positive by PCR assay with CPV-2ab primers. Higher incidence with PCR was probably due to detection of much lower levels of virus in feces (103 PFU/g) compared with HA.
KEY WORDS: Parvovirus, canine, agglutination, PCR.