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OJVRTM
Online Journal of Veterinary Research©
(Including Medical and Laboratory Research)
Established 1994
ISSN 1328-925X
Volume 25 (9):666-671, 2021.
Incidence of
canine parvovirus by hemagglutination, hemagglutination inhibition and PCR tests.
S Parthiban, HK Mukhopadhyay, K Ravikumar, PX Antony and RM
Pillai
Department of Veterinary Microbiology,
Rajiv Gandhi College of Veterinary and Animal Sciences, Puducherry-605 009.
SUMMARY
Parthiban S, Mukhopadhyay HK, Ravikumar K, Antony
PX, Pillai RM., Incidence of canine parvovirus by hemagglutination,
hemagglutination inhibition and PCR tests, Onl J Vet Res., 25 (9):666-671, 2021. One hundred twenty eight fecal and rectal samples were
taken from dogs with diarrhea and/or enteritis. The samples were clarified,
processed and subjected to hemagglutination (HA), hemagglutination
inhibition (HI) and polymerase chain reaction (PCR) for canine parvovirus
(CPV). By HA, 47/128 (36.7%) samples had titers 1:32 and above positive for CPV
but 81 (63.29%) had weak positive titers below 1:32. Highly positive CPV titers
were 1:64 and above whereas weak samples <1:32. Of 47 positive samples, 36
were highly positive and 11 weak positive. By hemagglutination
inhibition test (HI) with CPV hyper immune rabbit serum all 47 were inhibited.
By PCR we found 47 (36.7%) positive
for CPV infections by HA assay confirmed by hem-agglutination inhibition tests. 68 (53.1%) samples were found to be positive by PCR assay
with CPV-2ab primers. Higher incidence with PCR was probably due to detection
of much lower levels of virus in feces (103
PFU/g) compared with HA.
KEY WORDS: Parvovirus, canine, agglutination, PCR.
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