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Online Journal of Veterinary Research©
(Including Medical and Laboratory Research)
Volume 26 (3): 227-235, 2022.
Prevalence of canine enteritis parvovirus by hemagglutination and PCR tests.
S Parthiban, HK Mukhopadhyay, K Ravikumar, PX Antony, RM Pillai
Department of Veterinary Microbiology, Rajiv Gandhi College of Veterinary and Animal Sciences, Puducherry-605 009.
Parthiban S, Mukhopadhyay HK, Ravikumar K, Antony PX, Pillai RM., Prevalence of canine enteritis parvovirus by hemagglutination and PCR tests, Onl J Vet Res., 26 (3): 227-235, 2022. We collected swab fecal samples from 128 dogs with diarrhea/enteritis for canine parvovirus (CPV) tests. The samples were subjected to hemagglutination (HA) and polymerase chain reaction (PCR) assays. Of 128 samples, 47 (36.71%) had HA titre of >=1: 32 of which 36 had titres >=1.64 considered highly positive. HA results were confirmed on CPV rabbit hyperimmune serum yielding 47 positive samples. The same 128 samples were then subjected to PCR assay with CPV-2ab primer pair. We detected 68 (53.12%) yielding a single DNA amplicon of 681 bp to confirm CPV showing that 32 negative to HA were positive by PCR. Antibodies in intestinal lumen may bind virions to prevent hemagglutination whereas PCR can detect 103 virus PFU/gram of feces accounting for its higher sensitivity.
KEY WORDS: Canine, parvovirus, hemagglutination, PCR.