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OJVRTM
Online Journal of Veterinary Research©
Volume 16 (3):127-132, 2012. Redacted 2017.


HA-HI and PCR assays for detection of canine parvoviral enteritis.

 

S Parthiban*, HK Mukhopadhyay, K Ravikumar,  PX Antony and RM Pillai.

 

Department of Veterinary Microbiology, Rajiv Gandhi College of Veterinary and Animal Sciences, Puducherry India.

 

SUMMARY

 

Parthiban S, Mukhopadhyay HK, Ravikumar K,  Antony PX, Pillai RM., HA-HI and PCR assays for detection of canine parvoviral enteritis, Onl J Vet Res., 16 (3):127-132, 2012. A total of 128 clinical samples in the form of faecal samples/rectal swabs were collected from dogs showing signs of diarrhea/enteritis. The samples were clarified, processed and subjected to haemagglutination and polymerase chain reaction assays. Of the 128 samples screened 47 (36.71%) samples were positive for CPV infections by HA assay confirmed by haemagglutination inhibition tests. 68 (53.12%) samples were found to be positive by PCR assay with CPV-2ab primers.

 

KEY WORDS: CPV, HA-HI test, PCR assay.


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