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OJVRTM
Online Journal of Veterinary Research©
Volume 19(9):
698-703, 2015.
Effect of temperature and
disinfectants on Parascaris equorum
eggs.
Ehsan
Rakhshandehroo1, Mohsen Ghane2, Mohammad Asadpour1,
Arash Jafari1, Seyed
Hossein Malekpour1
1Department of Pathobiology
and 2Clinical Studies, School of Veterinary Medicine; Shiraz
University, Shiraz, Iran
Rakhshandehroo E, Ghane M, Asadpour M, Jafari A, Malekpour SH., Effect of temperature and disinfectants on Parascaris equorum eggs, Onl J Vet Res., 19(9): 698-703, 2015. Microtubes containing eggs of P. equorum were heated at 60, 70, 80 and 90ºC for 30 and 60 minutes and frozen at –20 and –70 ºC, for 1, 2, 3 and 5 hours and thawed at room temperature. Other samples of eggs were suspended in ethanol, formalin, sodium hydroxide and potassium dichromate solutions for 7 days. Viability of eggs was assessed in all groups and compared with eggs suspended in saline controls. Temperatures equal or higher than 60ºC for 30 minutes inactivated or killed P. equorum eggs. Temperatures of – 20ºC had no effect on egg viability but some did not survive at – 70ºC for 30 minutes. Ethanol and formaldehyde solutions at different concentrations reduced the percentage of viable eggs after 1 week of exposure but concentrated ethanol inactivated all eggs. Sodium hypochlorite, sodium hydroxide or potassium dichromate had no effect on eggs.
Keywords: Parascaris
equorum, viability, eggs, temperature.
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