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OJVRTM

Online Journal of Veterinary Research

 

Volume 23 (10):1032-1037, 2019.


Molecular and epidemiological study of pan-leukopenia in cats.

 

Amanee Mohammad Radhy and Mohammad Mushgil Zenad.

 

Department of Preventive Veterinary Medicine, College of Veterinary Medicine, University of Baghdad, Iraq.

 

ABSTRACT

 

Radhy AM, Zenad MM., Molecular and epidemiological study of feline pan-leukopenia, Onl J Vet Res., 23 (10):1032-1037, 2019. Feline pan-leukopenia (FPL) viruses cause high mortality in immuno-competent kittens and adult cats. We report incidence and epidemiology of FPL by PCR over a 1 year period in 100 domestic and 80 stray cats in Iraq. Feces and blood samples as well as swabs from mouth and eye lesions were tested for FPL viruses by PCR. PCR products were amplified at 250 base pairs with sequencing for VP2 gene in FPL virus by specific primers. The viruses showed homology to Gene Bank accession number MG 9774962 suggesting isolates in Iraq are of variable origin. In feces we detected virus~24% , in blood ~21%, mouth ~33% , eye ~32%, young 16-18%, adult 26-29%, domestic 23-27%, strays 19-20%, during cold months ~53%, temperate ~17% but hot months only 2 % (P ≤ 0.05).

 

Key words: Pan-leukemia, cats, PCR, Incidence, Iraq.


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