©1994-2018 All Rights Reserved. Online Journal of Veterinary Research. You
may not store these pages in any form except for your own personal use. All
other usage or distribution is illegal under international copyright
treaties. Permission to use any of these
pages in any other way besides the before mentioned must be gained in writing
from the publisher. This article is exclusively copyrighted
in its entirety to OJVR publications. This article may be copied once but may
not be, reproduced or re-transmitted without the express permission of the
editors.
OJVRTM
Online Journal of Veterinary Research©
Volume 22 (3):201-209, 2018.
Semen and sperm characteristics, genotype
mutations and allele frequency in Osteopontin gene
from Holstein bulls collected at different seasons.
Mohammad Yousif Mahmood1, Saad Akram Hatif1,Faris Faisel Ibrahim2, Wafa'a
Yeedam Lateef1
1College of Veterinary
Medicine, University of Baghdad, 2Ministry of Agriculture, Artificial Insemination
Center–Baghdad, Iraq.
ABSTRACT
Mahmood MY, Hatif SA, Ibrahim FF, Lateef
WY., Semen and sperm characteristics, genotype mutations and allele frequency
in Osteopontin gene from Holstein bulls collected at
different seasons, Onl J Vet Res., 22 (3):201-209,
2018. Semen
of 22 Holstein bulls collected during winter, spring and autumn was evaluated
for sperm concentration, volume, mass activity, and individual motility,
cooling and freezing. DNA in semen was extracted for PCR amplification of osteopontin (OPN) gene, between bp
4816 and 5528 of promoter region by sequence analysis. Our results revealed 5
genotype mutations and allele frequencies. Mutation rs109637038 had genotypes
GG and GA, rs110254070 CC, CT and TT, rs110329232 GG, GA and AA, rs109659827 CC
and CT, and rs380649619 GG, GA and AA. In mutation rs109637038, allele frequency
of G was 0.95 and A 0.84, in rs110329232, 0.84 and
0.05 and in rs380649619, 0.16 and 0.16, respectively. Frequencies of C and T in mutation rs110254070
were 0.93 and 0.77 and in rs109659827, 0.07 and 0.23, respectively. We found
significant (P<0.05) effect of season on mass and individual sperm motility.
Results suggest that polymorphisms in OPN gene and season of collection were related
with Holstein bull semen and sperm concentrations, volume, mass activity and
individual motility, and freezing characteristics.
Key words: Season, Sperm, Osteopontin
gene, Artificial insemination, bull.
FULL-TEXT (SUBSCRIPTION OR PURCHASE ARTICLE)