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Online Journal of Veterinary Research©
Volume 22 (3):201-209, 2018.
Semen and sperm characteristics, genotype mutations and allele frequency in Osteopontin gene from Holstein bulls collected at different seasons.
Mohammad Yousif Mahmood1, Saad Akram Hatif1,Faris Faisel Ibrahim2, Wafa'a Yeedam Lateef1
1College of Veterinary Medicine, University of Baghdad, 2Ministry of Agriculture, Artificial Insemination Center–Baghdad, Iraq.
Mahmood MY, Hatif SA, Ibrahim FF, Lateef WY., Semen and sperm characteristics, genotype mutations and allele frequency in Osteopontin gene from Holstein bulls collected at different seasons, Onl J Vet Res., 22 (3):201-209, 2018. Semen of 22 Holstein bulls collected during winter, spring and autumn was evaluated for sperm concentration, volume, mass activity, and individual motility, cooling and freezing. DNA in semen was extracted for PCR amplification of osteopontin (OPN) gene, between bp 4816 and 5528 of promoter region by sequence analysis. Our results revealed 5 genotype mutations and allele frequencies. Mutation rs109637038 had genotypes GG and GA, rs110254070 CC, CT and TT, rs110329232 GG, GA and AA, rs109659827 CC and CT, and rs380649619 GG, GA and AA. In mutation rs109637038, allele frequency of G was 0.95 and A 0.84, in rs110329232, 0.84 and 0.05 and in rs380649619, 0.16 and 0.16, respectively. Frequencies of C and T in mutation rs110254070 were 0.93 and 0.77 and in rs109659827, 0.07 and 0.23, respectively. We found significant (P<0.05) effect of season on mass and individual sperm motility. Results suggest that polymorphisms in OPN gene and season of collection were related with Holstein bull semen and sperm concentrations, volume, mass activity and individual motility, and freezing characteristics.
Key words: Season, Sperm, Osteopontin gene, Artificial insemination, bull.