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Online Journal of Veterinary Research

(Including Medical and Laboratory Research)

Established 1994
ISSN 1328-925X


Volume 26 (8): 644-650, 2022.

Effect of gonadotropins and frozen-thawed media on In vitro ewe embryo oocytes



SM Mirshamsi1, H Karami-Shabankareh1, J Habibizad2


1Department of Animal Science, Razi University, Kermanshah,2Animal Science, Faculty of Agriculture, Yasouj University, Iran.




Mirshamsi SM, Karami-Shabankareh H, Habibizad J., Effect of gonadotropins and frozen-thawed media on In vitro ewe embryo oocytes, Onl J Vet Res., Volume 26 (8): 644-650, 2022. We describe effects of gonadotropins and frozen-thawed media on ewe oocytes and zygotes. Oocytes were collected from sheep ovaries during non-breeding season for culture in media with 0.02 IU/mL pituitary, 0.075 IU/mL recombinant and/or 10 IU/mL chorionic gonadotropins or in frozen-thawed media for zygotes. Our results showed that embryo cleavage in P-FSH (61.1%) was lower (P<0.05) than rFSH-rLH (77.5%) and eCG-hCG (79.6%) We found no difference with blastocysts between pituitary (21.1%), recombinant (17.3%) and chorionic (20.5%) gonadotropins. Concerning 6 month storage and thawing our findings suggest that fresh or frozen storage media had no effect on blastocyst in P-FSH (21.1%), rFSH-rLH (17.3%) or eCG-hCG (20.5%), cleavage 73.2, 73.9, 77.4 or 78.2%, morula 42.0 44.3, 42.8 and 48.2% or in blastocysts 17.7, 17.1, 18.2 and 18.5%, respectively. We find that non-pituitary gonadotropins and frozen-thawed culture media support subsequent embryonic development.


Key words: immature oocytes, maturation medium, zygotes, culture medium, brilliant cresyl blue