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 Online Journal of Veterinary Research©

Volume 22 (8):645-649, 2018.

5’-Nucleotidase in sera of ewes with Brucellosis


Wisam  Mahmood Mohammed, Mustafa Salah Hasan, Noor Khalid Zaidan.


University of Fallujah - College of Veterinary Medicine, Fallujah, Iraq.




Mohammed WM, Hasan MS, Zaidan NK. 5’-Nucleotidase in sera of ewes with Brucellosis, Onl J Vet Res., 22 (8):645-649, 2018. We measured 5ʹ-nucleotidase (5-NT) in sera of 34-40 ewes with brucellosis, treated with or without 25 mg/kg streptomycin IM every 48 hours for 16 days. Controls (n=30) consisted of normal sheep. 5-NT catalyzes 5ʹ-ribonucleotides to the corresponding phosphate and ribonucleoside and plays an important role in adenosine metabolism in healthy sheep. Brucellosis was diagnosed by Rose–bengal and 2-mercaptoethanol tests and 5-NT as described by Wood and Williams (2001). Compared with controls (13.8 ± 3.31 IU/L) we found highly significant (P < 0.001) increases in 5-NT in ewes with brucellosis (46.4 ± 3.25 IU/L) but less (P < 0.05) in those given streptomycin (21.7 ± 2.80 UI/L). There were no differences in total serum protein between any group. Results suggest that 5ʹ-NT may reflect inflammation status in ewes with brucellosis.


Key Words: 5’-Nucleotidase, Sera, Ewes, Brucellosis.